LOCUS       sequence-324477-       12738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant binary expression vector containing the intron and proximal 
            polyadenylation signal sequences of the Copia93 retroelement EVADE 
            (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Oberlin S, Sarazin A, Chevalier C, Voinnet O, Mari-Ordonez A
  TITLE     A genome-wide transcriptome and translatome analysis of Arabidopsis 
            transposons identifies a unique and conserved genome expression 
            strategy for Ty1/Copia retroelements.
  JOURNAL   Genome Res. 2017 Sep;27(9):1549-1562. doi: 10.1101/gr.220723.117. 
            Epub 2017 Aug 7.
  PUBMED    28784835
REFERENCE   2  (bases 1 to 12738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.220723.117"; journalName: "Genome Res"; date: "2017-09";
            volume: "27"; issue: "9"; pages: "1549-1562"
COMMENT     Sequence Label: p35S_GFP-EVDinter-GUS
FEATURES             Location/Qualifiers
     source          1..12738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..33
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     15..32
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        680..1024
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     968..988
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1133..1157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1188..1216)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1811..1831
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             2712..4523
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     protein_bind    complement(4524..4548)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4561..4570
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        4964..5143
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5185..5736
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      5756..6011
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6104..6128
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6656..7447
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7691..8279
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8180..8199
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8433..8450
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8465..8605
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8531..8550)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8691..8713
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8949..9143
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9209..10276)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(10710..11339)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12639..12663
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    join(12732..12738,1..14)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gtatagaaaa gttgatttag gtgacactat agaatactca agctatgcat ccaacgcgtt
       61 gggagctctc ccatatggtc gagatctcct ttgccccgga gatcaccatg gacgactttc
      121 tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca
      181 ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta
      241 gagaggccta cgcggcaggt ctcatcaaga cgatctaccc gagtaataat ctccaggaga
      301 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      361 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      421 aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc
      481 ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg
      541 tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct
      601 tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
      661 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      721 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
      781 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
      841 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      901 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      961 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     1021 ggactgcagg acgatccgta tttttacaac aattaccaca acaaaacaaa caacaaacaa
     1081 cattacaatt tactattcta gtcgacctgc aggcggccgc actagtgata tcacaagttt
     1141 gtacaaaaaa gcaggctatg agtaaaggag aagaactttt cactggagtt gtcccaattc
     1201 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     1261 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     1321 ttccatggcc aaccctggtc accaccctga cctacggcgt gcagtgcttc tcccgttacc
     1381 ctgatcatat gaagcggcac gacttcttca agagcgccat gcctgaggga tacgtgcagg
     1441 agaggaccat cttcttcaag gacgacggga actacaagac acgtgctgaa gtcaagtttg
     1501 agggagacac cctcgtcaac aggatcgagc ttaagggaat cgatttcaag gaggacggaa
     1561 acatcctcgg ccacaagttg gaatacaact acaactccca caacgtatac atcatggccg
     1621 acaagcaaaa gaacggcatc aaagccaact tcaagacccg ccacaacatc gaagacggcg
     1681 gcgtgcaact cgctgatcat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1741 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     1801 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggtag
     1861 aactatacaa actcgaggag acatccacat cattcggaga ctatgtgagg aagtcggact
     1921 tggaagcact catcaagtcc attgcttcac tcaaagaatc cggtatcact ttctcatcac
     1981 aaacatctag tagttcaatt gtgatagact cgggtgcttc tcatcatatg attagtaact
     2041 ctaatcttct tgataacata gaaccagctc taggacatgt tattattgct aatggtgaca
     2101 aagttccaat tgaagggata ggaaacttga aattgtttaa caaagattca aaagctttct
     2161 ttatgcctaa gttcacatct aacttattat ccgtgaaaag gaccactaga gacttaaatt
     2221 gttatgcgat ttttggccct aatgatgtgt attttcagga tattgagacg ggaaaagtta
     2281 ttggagaagg tggttcaaaa ggagaactct atgttcttga agacttgtca ccaaactctt
     2341 ctagctgttt ttcctctaag tcacatttag gcatctcttt taatactttg tggcatgcta
     2401 ggctaggtca tcctcatact agagctctta aactcatgtt accaaatatt tcttttgatc
     2461 atacaagttg tgaggcttgt attcttggta aacattgcaa atcggttttt cctaaatctc
     2521 ttactatcta tgagaaatgt tttgatctag ttcattcgga tgtttggacc tcaccatgtg
     2581 tttctagaga caacaacaaa tattttgtca catttattga tgagaaatca aagtacactt
     2641 ggataacact attaccctca aaagatagag tgtttgaagc tttcactaac tttgaaacct
     2701 atgtccctag gatgttacgt cctgtagaaa ccccaacccg tgaaatcaaa aaactcgacg
     2761 gcctgtgggc attcagtctg gatcgcgaaa actgtggaat tgatcagcgt tggtgggaaa
     2821 gcgcgttaca agaaagccgg gcaattgctg tgccaggcag ttttaacgat cagttcgccg
     2881 atgcagatat tcgtaattat gcgggcaacg tctggtatca gcgcgaagtc tttataccga
     2941 aaggttgggc aggccagcgt atcgtgctgc gtttcgatgc ggtcactcat tacggcaaag
     3001 tgtgggtcaa taatcaggaa gtgatggagc atcagggcgg ctatacgcca tttgaagccg
     3061 atgtcacgcc gtatgttatt gccgggaaaa gtgtacgtat caccgtttgt gtgaacaacg
     3121 aactgaactg gcagactatc ccgccgggaa tggtgattac cgacgaaaac ggcaagaaaa
     3181 agcagtctta cttccatgat ttctttaact atgccggaat ccatcgcagc gtaatgctct
     3241 acaccacgcc gaacacctgg gtggacgata tcaccgtggt gacgcatgtc gcgcaagact
     3301 gtaaccacgc gtctgttgac tggcaggtgg tggccaatgg tgatgtcagc gttgaactgc
     3361 gtgatgcgga tcaacaggtg gttgcaactg gacaaggcac tagcgggact ttgcaagtgg
     3421 tgaatccgca cctctggcaa ccgggtgaag gttatctcta tgaactgtgc gtcacagcca
     3481 aaagccagac agagtgtgat atctacccgc ttcgcgtcgg catccggtca gtggcagtga
     3541 agggcgaaca gttcctgatt aaccacaaac cgttctactt tactggcttt ggtcgtcatg
     3601 aagatgcgga cttgcgtggc aaaggattcg ataacgtgct gatggtgcac gaccacgcat
     3661 taatggactg gattggggcc aactcctacc gtacctcgca ttacccttac gctgaagaga
     3721 tgctcgactg ggcagatgaa catggcatcg tggtgattga tgaaactgct gctgtcggct
     3781 ttaacctctc tttaggcatt ggtttcgaag cgggcaacaa gccgaaagaa ctgtacagcg
     3841 aagaggcagt caacggggaa actcagcaag cgcacttaca ggcgattaaa gagctgatag
     3901 cgcgtgacaa aaaccaccca agcgtggtga tgtggagtat tgccaacgaa ccggataccc
     3961 gtccgcaagg tgcacgggaa tatttcgcgc cactggcgga agcaacgcgt aaactcgacc
     4021 cgacgcgtcc gatcacctgc gtcaatgtaa tgttctgcga cgctcacacc gataccatca
     4081 gcgatctctt tgatgtgctg tgcctgaacc gttattacgg atggtatgtc caaagcggcg
     4141 atttggaaac ggcagagaag gtactggaaa aagaacttct ggcctggcag gagaaactgc
     4201 atcagccgat tatcatcacc gaatacggcg tggatacgtt agccgggctg cactcaatgt
     4261 acaccgacat gtggagtgaa gagtatcagt gtgcatggct ggatatgtat caccgcgtct
     4321 ttgatcgcgt cagcgccgtc gtcggtgaac aggtatggaa tttcgccgat tttgcgacct
     4381 cgcaaggcat attgcgcgtt ggcggtaaca agaaagggat cttcactcgc gaccgcaaac
     4441 cgaagtcggc ggcttttctg ctgcaaaaac gctggactgg catgaacttc ggtgaaaaac
     4501 cgcagcaggg aggcaaacaa tgaacccagc tttcttgtac aaagtggtga tatcccgcgg
     4561 ccatggcggc cgggagcatg cgacgtcgat ctaactgact agccgcggcc atgctagagt
     4621 ccgcaaaaat caccagtctc tctctacaaa tctatctctc tctatttttc tccagaataa
     4681 tgtgtgagta gttcccagat aagggaatta gggttcttat agggtttcgc tcatgtgttg
     4741 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
     4801 tctaattcct aaaaccaaaa tccagtgacc tgcaggcatg cgacgtcggg ccctctagag
     4861 gatccccggg taccctcgaa ttatcataca tgagaattaa gggagtcacg ttatgacccc
     4921 cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag
     4981 gagccactga gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat
     5041 tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa
     5101 atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc
     5161 tcaactcgat cgaggggatc taccatgagc ccagaacgac gcccggccga catccgccgt
     5221 gccaccgagg cggacatgcc ggcggtctgc accatcgtca accactacat cgagacaagc
     5281 acggtcaact tccgtaccga gccgcaggaa ccgcaggagt ggacggacga cctcgtccgt
     5341 ctgcgggagc gctatccctg gctcgtcgcc gaggtggacg gcgaggtcgc cggcatcgcc
     5401 tacgcgggtc cctggaaggc acgcaacgcc tacgactgga cggccgagtc gaccgtgtac
     5461 gtctcccccc gccaccagcg gacgggactg ggctccacgc tctacaccca cctgctgaag
     5521 tccctggagg cacagggctt caagagcgtg gtcgctgtca tcgggctgcc caacgacccg
     5581 agcgtgcgca tgcacgaggc gctcggatat gccccccgcg gcatgctgcg ggcggccggc
     5641 ttcaagcacg ggaactggca tgacgtgggt ttctggcagc tggacttcag cctgccggtg
     5701 ccgccccgtc cggtcctgcc cgtcaccgaa atctgatgac ccctagagtc aagcagatcg
     5761 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     5821 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     5881 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     5941 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctactat
     6001 gttactagat cgaccggcat gcaagctgat aattcaattc ggcgttaatt cagtacatta
     6061 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
     6121 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
     6181 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
     6241 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
     6301 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
     6361 tgatcaattc gggcacgaac ccagtggaca taagcctcgt tcggttcgta agctgtaatg
     6421 caagtagcgt aactgccgtc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     6481 aacggcgcag tggcggtttt catggcttct tgttatgaca tgtttttttg gggtacagtc
     6541 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     6601 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg
     6661 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     6721 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     6781 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     6841 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     6901 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     6961 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     7021 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     7081 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     7141 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     7201 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     7261 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     7321 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     7381 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     7441 caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgccggc gttaactcaa
     7501 gcgattagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     7561 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
     7621 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7681 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7741 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7801 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     7861 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7921 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7981 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     8041 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     8101 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8161 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8221 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8281 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8341 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8401 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8461 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     8521 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     8581 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     8641 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     8701 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
     8761 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
     8821 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
     8881 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
     8941 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
     9001 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
     9061 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
     9121 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
     9181 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
     9241 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
     9301 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
     9361 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
     9421 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
     9481 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
     9541 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
     9601 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
     9661 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
     9721 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
     9781 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
     9841 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
     9901 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
     9961 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
    10021 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
    10081 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
    10141 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
    10201 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
    10261 cggatggttt gcgaccgtca cgccgattcc tcgggcttgg gggttccagt gccattgcag
    10321 ggccggcaga caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg
    10381 cattccacgg cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct
    10441 aaaattcatc tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc
    10501 agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg
    10561 atcctccgcc ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc
    10621 caacgttgca gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct
    10681 tttgctcatt ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc
    10741 agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg
    10801 gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc
    10861 tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc
    10921 gacacgacaa aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc
    10981 tccaccaggt cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg
    11041 aagtcggctg ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc
    11101 actacgaagt cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg
    11161 ccgacaacgg ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg
    11221 ggatcggaat cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg
    11281 gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg
    11341 cgttcccctt gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac
    11401 gcaagctgtt ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag
    11461 cggccaagct ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat
    11521 gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct
    11581 ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca
    11641 tgcttgttcc tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc
    11701 gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc
    11761 aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt
    11821 gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg
    11881 gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc
    11941 gatgattagg gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc
    12001 ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc
    12061 ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct
    12121 ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg
    12181 gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag
    12241 ttcggcccgt tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag
    12301 gccagcggcg gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta
    12361 ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg
    12421 tcgcgtaact taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca
    12481 gaagccgact gcactatagc agcggagggg ttggatcaaa gtactttgat cccgagggga
    12541 accctgtggt tggcatgcac atacaaatgg acgaacggat aaaccttttc acgccctttt
    12601 aaatatccgt tattctaata aacgctcttt tctcttaggt ttacccgcca atatatcctg
    12661 tcaaacactg atagtttaaa ctgaaggcgg gaaacgacaa tctgatccaa gctcaagcta
    12721 agcttgagct ccaacttt
//
