LOCUS       sequence-324517-        2263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPKK0CM0107
FEATURES             Location/Qualifiers
     source          1..2263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(618..712)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(733..1392)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1307..1326
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1554..1572
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1576..1619)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1858..1915
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1939..1956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2110..2263,1..435))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2190..2209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
       61 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      121 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      181 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      241 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      301 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      361 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      421 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      481 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      541 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      601 gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
      661 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
      721 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
      781 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
      841 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
      901 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
      961 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1021 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1081 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     1141 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     1201 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     1261 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     1321 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     1381 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     1441 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc
     1501 gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1561 gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct
     1621 ggaattcggt ctcaggaggt tatcacactt ggtaatttag aagaagaaac ccataggttc
     1681 tgtgggtttt ttgggtagaa gaagaaaccc ataggttctg tgggtttttt gggtagaaga
     1741 agaaacccat aggttctgtg ggttttttgg gtagaagaag aaacccatag ctatataatg
     1801 agaaacaggg gcaaatcccc gaaaacaaat aacgcgaagc aatgcgagac cctgcagtcc
     1861 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact
     1921 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1981 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2041 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2101 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2161 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2221 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tac
//
