LOCUS       sequence-324520-        2303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPKK0CM0065
FEATURES             Location/Qualifiers
     source          1..2303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(92..111)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(783..877)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(898..1557)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1472..1491
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1719..1737
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1741..1784)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    1816..1849
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    1875..1908
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        1925..1971
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      2063..2120
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2144..2161)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
       61 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      121 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      181 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      241 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      301 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      361 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      421 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      481 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      541 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      601 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      661 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      721 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
      781 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
      841 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
      901 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
      961 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     1021 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     1081 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     1141 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     1201 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     1261 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     1321 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     1381 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     1441 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     1501 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     1561 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     1621 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg
     1681 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     1741 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcggtctcag
     1801 gagggagcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccactcc
     1861 ccgagccggt gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccactccc
     1921 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg actccggtat
     1981 ttttacaaca attaccacaa caaaacaaac aacaaacaac attacaattt actattctag
     2041 tcgaaaatgc gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt
     2101 tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc
     2161 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2221 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2281 ccaggaaccg taaaaaggcc gcg
//
