LOCUS       sequence-324527-        9781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ASCL1 under the control of the TetON 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Black JB, McCutcheon SR, Dube S, Barrera A, Klann TS, Rice GA, Adkar
            SS, Soderling SH, Reddy TE, Gersbach CA
  TITLE     Master Regulators and Cofactors of Human Neuronal Cell Fate 
            Specification Identified by CRISPR Gene Activation Screens.
  JOURNAL   Cell Rep. 2020 Dec 1;33(9):108460. doi: 
            10.1016/j.celrep.2020.108460.
  PUBMED    33264623
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-12-1"; volume: "33(9):108460. doi"; pages: " 
            10.1016/j.celrep.2020.108460"
COMMENT     Sequence Label: pTet-O-ASCL1-T2A-PuroR
FEATURES             Location/Qualifiers
     source          1..9781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2..55
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             62..661
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(62..81)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     558..578
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    700..1288
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(753..773)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1171..1182)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1291..1307)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1292..1308)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1813..1993
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2019..2036)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2025..2249
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2295..2723
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2382..2401)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2592..2613
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2732..2752)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2737..3066
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2917..3052
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2979..2998
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3114..3161
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3180..3554
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3684..3805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3721..3740)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3775..3794
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3854..3870)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3854..3870)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3867..3889)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3878..3894
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3902..3908,3909..3926,3927..3932))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3947..3968
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4085..4102)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4256..4844)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4336..4355)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5015..5806,5807..5875))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5638..5657
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5876..5980)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6055..6074)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6246..6625
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6627..6825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             6843..7023
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7070..7195
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7688..7921
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8106..8150
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8448..8565
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(8622..8640,8641..8663,8664..8682,8683..8705,8706..8724,
                     8725..8747,8748..8766,8767..8789,8790..8808,8809..8831,
                     8832..8850,8851..8873,8874..8892)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        8925..8963
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8960..8984
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      9063..9072
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agagggcagg ggaagtcttc taacatgcgg ggacgtggag gaaaatcccg gcccactcga
       61 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
      121 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
      181 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
      241 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
      301 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
      361 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
      421 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
      481 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
      541 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
      601 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
      661 attaattaag ctagctaatt cgatatcaag cttatcgata atcaacctct ggattacaaa
      721 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
      781 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
      841 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
      901 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
      961 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     1021 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     1081 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     1141 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     1201 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     1261 cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga cctagaaaaa
     1321 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
     1381 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
     1441 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     1501 ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     1561 tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt
     1621 ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga
     1681 gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     1741 gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat
     1801 ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     1861 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     1921 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1981 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     2041 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     2101 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     2161 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     2221 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     2281 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     2341 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     2401 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     2461 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     2521 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     2581 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     2641 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     2701 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     2761 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     2821 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     2881 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2941 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3001 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     3061 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca
     3121 attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca
     3181 tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg
     3241 agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg
     3301 tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca
     3361 acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg
     3421 tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc
     3481 cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg
     3541 aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt
     3601 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     3661 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     3721 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     3781 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     3841 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3901 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3961 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4021 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4081 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4141 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4201 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4261 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4321 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4381 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4441 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4501 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4561 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     4621 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     4681 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4741 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4801 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4861 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4921 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4981 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5041 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5101 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5161 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5221 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5281 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5341 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5401 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5461 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5521 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5581 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     5641 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5701 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5761 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5821 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5881 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5941 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6001 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     6061 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     6121 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     6181 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     6241 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     6301 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     6361 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     6421 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     6481 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     6541 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     6601 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     6661 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     6721 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     6781 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
     6841 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6901 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6961 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7021 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     7081 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     7141 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     7201 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     7261 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     7321 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     7381 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     7441 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     7501 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     7561 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     7621 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     7681 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     7741 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     7801 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     7861 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     7921 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     7981 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     8041 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     8101 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     8161 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     8221 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     8281 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     8341 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     8401 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     8461 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     8521 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     8581 gacagcagag atccagtttg gttagatctc gagtttacca ctccctatca gtgatagaga
     8641 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     8701 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     8761 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     8821 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     8881 cagtgataga gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg cgtgtacggt
     8941 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc
     9001 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat
     9061 tcgccaccat ggaaagctct gccaagatgg agagcggcgg cgccggccag cagccccagc
     9121 cgcagcccca gcagcccttc ctgccgcccg cagcctgttt ctttgccacg gccgcagccg
     9181 cggcggccgc agccgccgca gcggcagcgc agagcgcgca gcagcagcag cagcagcagc
     9241 agcagcagca gcaggcgccg cagctgagac cggcggccga cggccagccc tcagggggcg
     9301 gtcacaagtc agcgcccaag caagtcaagc gacagcgctc gtcttcgccc gaactgatgc
     9361 gctgcaaacg ccggctcaac ttcagcggct ttggctacag cctgccgcag cagcagccgg
     9421 ccgccgtggc gcgccgcaac gagcgcgagc gcaaccgcgt caagttggtc aacctgggct
     9481 ttgccaccct tcgggagcac gtccccaacg gcgcggccaa caagaagatg agtaaggtgg
     9541 agacactgcg ctcggcggtc gagtacatcc gcgcgctgca gcagctgctg gacgagcatg
     9601 acgcggtgag cgccgccttc caggcaggcg tcctgtcgcc caccatctcc cccaactact
     9661 ccaacgactt gaactccatg gccggctcgc cggtctcatc ctactcgtcg gacgagggct
     9721 cttacgaccc gctcagcccc gaggagcagg agcttctcga cttcaccaac tggttctcta
     9781 g
//
