LOCUS       sequence-32453-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Dunlop MJ, Cox RS, Levine JH, Murray RM, Elowitz MB
  TITLE     Regulatory activity revealed by dynamic correlations in gene 
            expression noise.
  JOURNAL   Nat Genet. 2008 Dec . 40(12):1493-8.
  PUBMED    19029898
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2008 Dec . 40(12):1493-8."
COMMENT     Sequence Label: pNS2-sigmaVL
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     205..223
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    complement(229..262)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     RBS             330..341
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ctgcgtgcat ccatcttgtt catcatgcga aacgatcctc atcctgtctc ttgatcngat
       61 catgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
      121 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccgacgtcta agaaaccatt
      181 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttat aacttcgtat
      241 agcatacatt atacgaagtt atctcgagaa taattcttga catttatgct tccggctcgt
      301 ataatacgtg caattggatc cttaacttta aagaggagaa aggtaccgca tgactagcaa
      361 aagaagcaaa ggtgaagaac tgttcactgg tgttgttcca attctggttg aactggatgg
      421 tgatgttaat ggtcacaaat tttctgtctc tggtgagggt gaaggtgatg caacctacgg
      481 taaactgacc ctgaaattta tttgcactac tggtaaactg cctgttccgt ggccaaccct
      541 ggtcactact ctgacttggg gtgttcaatg ctttgctcgt tacccagatc acatgaaaca
      601 gcatgacttt ttcaagtctg ccatgccgga aggttatgtt caggaacgta ctatcttttt
      661 caaagatgac ggtaactaca agacccgtgc tgaagtcaag tttgaaggtg ataccctggt
      721 taatcgtatc gagctgaaag gtattgattt taaagaagat ggtaacattc tgggtcacaa
//
