LOCUS       sequence-324530-        2175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPKK0CM0063
FEATURES             Location/Qualifiers
     source          1..2175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(91..110)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(782..876)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(897..1556)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1471..1490
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1718..1736
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1740..1783)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    1811..1844
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    1870..1903
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     terminator      1934..1991
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2015..2032)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
       61 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      121 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      181 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      241 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      301 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      361 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      421 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      481 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
      541 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
      601 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
      661 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      721 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg
      781 gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga
      841 gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac
      901 gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg
      961 aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct
     1021 tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg
     1081 tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca
     1141 ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat
     1201 atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca
     1261 gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg
     1321 tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata
     1381 aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc
     1441 agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct
     1501 ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta
     1561 gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt
     1621 tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt
     1681 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa
     1741 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt cggtctcagg
     1801 agcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc actccccgag
     1861 ccggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca ctcccctgac
     1921 cgagaccctg cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa
     1981 aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg
     2041 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2101 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2161 gtaaaaaggc cgcgt
//
