LOCUS       sequence-324531-        2175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPKK0CM0064
FEATURES             Location/Qualifiers
     source          1..2175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(100..119)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(791..885)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(906..1565)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1480..1499
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1727..1745
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1749..1792)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    1820..1853
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    1879..1912
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     terminator      1943..2000
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2024..2041)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
       61 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      121 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      181 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      241 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      301 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      361 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      421 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      481 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      541 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      601 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      661 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      721 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
      781 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
      841 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
      901 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
      961 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1021 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1081 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     1141 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1201 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1261 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1321 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     1381 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1441 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1501 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     1561 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     1621 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
     1681 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     1741 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
     1801 ggtctcatga ccggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca
     1861 ctccccgagc cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
     1921 tccccccatc gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt
     1981 tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc
     2041 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2101 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2161 ccaggaaccg taaaa
//
