LOCUS       sequence-324533-        2149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPAS0CMR0015
FEATURES             Location/Qualifiers
     source          1..2149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(92..186)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(207..866)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     781..800
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1028..1046
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1050..1093)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1218..1275
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1299..1316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1470..2058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1550..1569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
       61 agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc
      121 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
      181 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
      241 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
      301 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
      361 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
      421 ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
      481 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
      541 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
      601 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
      661 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
      721 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
      781 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
      841 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
      901 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
      961 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     1021 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     1081 taaggatgat ttctggaatt cggtctcagg aggttctgtg ggttttttgg gtagaagaag
     1141 aaacccatag ctatataatg agaaacaggg gcaaatcccc gaaaacaaat aacgcgaagc
     1201 aatgcgagac cctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct
     1261 ttaaaaccga aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc
     1321 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1381 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1441 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat
     1501 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1561 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1621 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1681 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1741 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1801 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1861 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1921 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1981 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2041 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2101 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaagga
//
