LOCUS       sequence-324547-       14401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Brainbow transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14401)
  AUTHORS   Yoshimatsu S, Ohtsu K, Sato T, Yamamoto M, Sasaki E, Shiozawa S, 
            Okano H
  TITLE     Generation and validation of a common marmoset embryonic stem cell 
            line ActiCre-B1 that ubiquitously expresses a tamoxifen-inducible 
            Cre-driver.
  JOURNAL   Stem Cell Res. 2021 Jan 8;51:102164. doi: 10.1016/j.scr.2021.102164.
  PUBMED    33453576
REFERENCE   2  (bases 1 to 14401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2021-01-8"; volume: "51:102164. doi"; pages: " 
            10.1016/j.scr.2021.102164"
COMMENT     Sequence Label: pPB-CAG-Brainbow3.2-PGK-PuroTK
FEATURES             Location/Qualifiers
     source          1..14401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    35..242
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        405..904
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(440..463)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     837..856
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             928..1524
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(928..947)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1424..1444
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1531..2526
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     repeat_region   3052..3114
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(3138..3156)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3177..3193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3177..3193)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3190..3212)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3201..3217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3225..3231,3232..3249,3250..3255))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3270..3291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3408..3425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3579..4167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3659..3678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4338..5129,5130..5198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4961..4980
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5199..5303)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5329..5784)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5466..5487)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5678..5697
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5911..5933
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5925..5942
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5926..5942
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5949..5968
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5949..5967
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   5986..6020
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        6298..6681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          6964..7980
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     7904..7926
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     7988..8007
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    8056..8080
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8129..8162)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(8171..8204)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(8213..8246)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      8255..8264
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8267..8287
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8288..8992
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTAGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
     polyA_signal    9175..9296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(9309..9342)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      9351..9360
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9357..10064
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    10152..10740
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(10623..10634)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10874..10995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(11012..11045)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      11054..11063
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(11060..11062,11063..11065,11066..11776)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(11060..11062,11063..11065,11066..11776)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11105..11126)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11366..11385)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    11864..12452
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(12335..12346)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    12586..12707
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(12726..12759)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      12768..12777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12774..13469
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     misc_feature    13557..14145
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(14028..14039)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    14320..14353
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(join(14392..14401,1..15))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cttgtacaaa gtggccccga cctgcaggca tgcactgtgc cttctagttg ccagccatct
       61 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
      121 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
      181 ggtggggtgg ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg
      241 gattaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa
      301 tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc
      361 ttggttatgc cggtactgcc gggcctcttg cggggaattc taccgggtag gggaggcgct
      421 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac
      481 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt
      541 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc
      601 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt
      661 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata
      721 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt cccggggcgg
      781 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt
      841 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct
      901 ttcgaccgat catcaagctt gatcctcatg accgagtaca agcccacggt gcgcctcgcc
      961 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     1021 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     1081 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     1141 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     1201 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     1261 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     1321 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     1381 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     1441 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     1501 tgcatgaccc gcaagcccgg tgccggatcc atgcccacgc tactgcgggt ttatatagac
     1561 ggtccccacg ggatggggaa aaccaccacc acgcaactgc tggtggccct gggttcgcgc
     1621 gacgatatcg tctacgtacc cgagccgatg acttactggc gggtgctggg ggcttccgag
     1681 acaatcgcga acatctacac cacacaacac cgcctcgacc agggtgagat atcggccggg
     1741 gacgcggcgg tggtaatgac aagcgcccag ataacaatgg gcatgcctta tgccgtgacc
     1801 gacgccgttc tggctcctca tatcgggggg gaggctggga gctcacatgc cccgcccccg
     1861 gccctcaccc tcatcttcga ccgccatccc atcgccgccc tcctgtgcta cccggccgcg
     1921 cggtacctta tgggcagcat gaccccccag gccgtgctgg cgttcgtggc cctcatcccg
     1981 ccgaccttgc ccggcaccaa catcgtgctt ggggcccttc cggaggacag acacatcgac
     2041 cgcctggcca aacgccagcg ccccggcgag cggctggacc tggctatgct ggctgcgatt
     2101 cgccgcgttt acgggctact tgccaatacg gtgcggtatc tgcagtgcgg cgggtcgtgg
     2161 cgggaggact ggggacagct ttcggggacg gccgtgccgc cccagggtgc cgagccccag
     2221 agcaacgcgg gcccacgacc ccatatcggg gacacgttat ttaccctgtt tcgggccccc
     2281 gagttgctgg cccccaacgg cgacctgtat aacgtgtttg cctgggcctt ggacgtcttg
     2341 gccaaacgcc tccgttccat gcacgtcttt atcctggatt acgaccaatc gcccgccggc
     2401 tgccgggacg ccctgctgca acttacctcc gggatggtcc agacccacgt caccaccccc
     2461 ggctccatac cgacgatatg cgacctggcg cgcacgtttg cccgggagat gggggaggct
     2521 aactgagctc tagcttatcg actcgccgat agtggaaacc gacgccccag cactcgtccg
     2581 agggcaaagg aatagtcgag aaattgatga tctattaaac aataaagatg tccacatgga
     2641 agtttttcct gtcatacttt gttaagaagg gtgagaacag agtacctaca ttttgaatgg
     2701 aaggattgga gctacggggg tgggggtggg gtgggattag ataaatgcct gctctttact
     2761 gaaggctctt tactattgct ttatgataat gtttcatagt tggatatcat aatttaaaca
     2821 agcaaaacca aattaagggc cagctcattc ctcccactca tgatctatag atccatcaga
     2881 tcctttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata aataaataaa
     2941 cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat aataaaactt
     3001 aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac acatgcgtca
     3061 attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct agggttaaag
     3121 agctccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca
     3181 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     3241 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     3301 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     3361 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     3421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     4681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     5341 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     5401 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     5461 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     5521 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     5581 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     5641 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     5701 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     5761 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     5821 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     5881 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     5941 gtgaattgta atacgactca ctatagggcg aattgggtac cttaacccta gaaagatagt
     6001 ctgcgtaaaa ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa
     6061 tccgtcgctg tgcatttagg acatctcagt cgccgcttgg agctcccgtg aggcgtgctt
     6121 gtcaatgcgg taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc
     6181 atgattatct tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc
     6241 atgttctact tacgtgataa cttattatat atatattttc ttgttataga tatcgtcgac
     6301 attgattatt gactagctag ttattaatag taatcaatta cggggtcatt agttcatagc
     6361 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     6421 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     6481 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     6541 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     6601 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     6661 ttagtcatcg ctattaccat gatggtcgag gtgagcccca cgttctgctt cactctcccc
     6721 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     6781 gcgatggggg cggggggggg gggggggcgc gcgccggggg ggggggggsg gggsgggggg
     6841 sggggsgggg cgrggcggrg rggkgcggcg gcagccaatc agagcggcgc gctccgaaag
     6901 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     6961 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
     7021 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     7081 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     7141 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     7201 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     7261 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     7321 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     7381 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     7441 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     7501 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     7561 gcggggccgc cycgggccgg ggrgggctcg ggggrggggc gcggcggccc ccggagcgcc
     7621 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     7681 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     7741 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     7801 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     7861 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     7921 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     7981 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcctcgag
     8041 attttggcaa agaattcaag tttgtacaaa aaagcaggct ttaaaggaac gcacgtaact
     8101 ataacggtcc taaggtagcg aacctaggat aacttcgtat agcatacatt atacgaagtt
     8161 atcggcgcgc ataacttcgt ataggatact ttatacgaag ttatcaagcg ctataacttc
     8221 gtatagtata ccttatacga agttatctga tatcgccacc atggctccta agaagaagag
     8281 gaaggtgatg agcagcggcg ccctgctgtt ccacggcaag atcccctacg tggtggagat
     8341 ggagggcaat gtggatggcc acaccttcag catccgcggc aagggctacg gcgatgccag
     8401 cgtgggcaag gtggatgccc agttcatctg caccaccggc gatgtgcccg tgccctggag
     8461 caccctggtg accaccctga ccgcaggcgc ccagtgcttc gccaagtacg gccccgagct
     8521 gaaggatttc tacaagagct gcatgcccga tggctacgtg caggagcgca ccatcacctt
     8581 cgagggtgat ggcaatttca agacccgcgc cgaggtgacc ttcgagaatg gcagcgtgta
     8641 caatcgcgtg aagctgaatg gccagggctt caagaaggat ggccacgtgc tgggcaagaa
     8701 tctggagttc aatttcaccc cccactgcct gtacatctgg ggcgatcagg ccaatcacgg
     8761 cctgaagagc gccttcaaga tctgccacga gatcaccggc agcaagggcg atttcatcgt
     8821 ggccgatcac acccagatga atacccccat cggcggcggc cccgtgcacg tgcccgagta
     8881 ccaccacatg agctaccacg tgaagctgag caaggatgtg accgatcacc gcgataatat
     8941 gagcctgaag gagaccgtgc gcgccgtgga ttgccgcaag acctacctgt gaagcttaat
     9001 tagctgagct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggccg
     9061 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     9121 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     9181 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9241 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
     9301 gtgctagcat aacttcgtat agcatacatt atacgaagtt atctaacgtt gccaccatgg
     9361 tgagcaaggg cgaggagaat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     9421 gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     9481 cctacgaggg ctttcagacc gctaagctga aggtgaccaa gggtggcccc ctgcccttcg
     9541 cctgggacat cctgtcccct catttcacct acggctccaa ggcctacgtg aagcaccccg
     9601 ccgacatccc cgactacttc aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     9661 tgaactacga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     9721 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtgatgc
     9781 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggtgccc
     9841 tgaagggcaa gatcaagatg aggctgaagc tgaaggacgg cggccactac acctccgagg
     9901 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca
     9961 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
    10021 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagagtgca cgcgtgagta
    10081 agctgaaccc tcctgatgag agtggccccg gctgcatgag ctgcaagtgt gtgctctcct
    10141 gagcggccgc taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
    10201 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
    10261 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
    10321 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
    10381 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
    10441 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
    10501 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
    10561 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
    10621 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
    10681 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
    10741 atcgataccg tcgagactgc tgcaccagat cataatcagc cataccacat ttgtagaggt
    10801 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
    10861 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10921 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10981 catcaatgta tcttaaggcg tgtttaaaca tataacttcg tataggatac tttatacgaa
    11041 gttatcgcgt acggccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
    11101 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
    11161 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
    11221 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
    11281 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
    11341 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
    11401 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
    11461 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
    11521 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
    11581 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
    11641 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
    11701 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
    11761 ggacgagctg tacaagagtg cacgcgtgag taagctgaac cctcctgatg agagtggccc
    11821 cggctgcatg agctgcaagt gtgtgctctc ctgagcggcc gctaatcaac ctctggatta
    11881 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
    11941 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
    12001 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
    12061 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
    12121 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
    12181 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
    12241 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
    12301 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
    12361 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
    12421 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgagact gctgcaccag
    12481 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
    12541 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
    12601 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    12661 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtgaagatc
    12721 ttcatataac ttcgtatagt ataccttata cgaagttatc aagtactgcc accatggtga
    12781 gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtgaacaacc
    12841 accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
    12901 gaatcaaggc ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctaccagct
    12961 tcatgtacgg cagcaaaacc ttcatcaacc acacccaggg catccccgac ttctttaagc
    13021 agtccttccc cgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
    13081 tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca
    13141 gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
    13201 cctccaccga gaccctgtac cccgctgacg gcggcctgga aggcagagcc gacatggccc
    13261 tgaagctcgt gggcgggggc cacctgatct gcaacttgaa gaccacatac agatccaaga
    13321 aacccgctaa gaacctcaag atgcccggcg tctactatgt ggacagaaga ctggaaagaa
    13381 tcaaggaggc cgacaaagag acctacgtcg agcagcacga ggtggctgtg gccagatact
    13441 gcgacctccc tagcaaactg gggcacagaa gtgcacgcgt gagtaagctg aaccctcctg
    13501 atgagagtgg ccccggctgc atgagctgca agtgtgtgct ctcctgagcg gccgctaatc
    13561 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
    13621 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
    13681 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
    13741 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
    13801 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
    13861 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
    13921 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
    13981 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
    14041 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
    14101 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgag
    14161 actgctgcac cagatcataa tyrkymacty gtttattgca gmtkwtaatg gttacaaata
    14221 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    14281 tttgtccaaa ctcatcaatg tatcttaagg cgtactagtg aagttcctat tctctagaaa
    14341 gtataggaac ttccgtaact ataacggtcc taaggtagcg aatgcatcta gacccagctt
    14401 t
//
