LOCUS       sequence-324552-        9176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral overexpression vector for human NADK .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Rossiter NJ, Huggler KS, Adelmann CH, Keys HR, Soens RW, Sabatini 
            DM, Cantor JR
  TITLE     CRISPR screens in physiologic medium reveal conditionally essential 
            genes in human cells.
  JOURNAL   Cell Metab. 2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005.
  PUBMED    33651980
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Metab.
            2021 Feb 23. pii: S1550-4131(21)00061-9. doi: 
            10.1016/j.cmet.2021.02.005."
COMMENT     Sequence Label: pLJC6-NADK-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1824..3035
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2872..2891
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4430..4495
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4543..4660
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4709..5219
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5138..5156
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5228..5623
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5695..5928
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6000..6121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6037..6056)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6091..6110
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6161..6296
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6316..6335)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6317..6335)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6345..6362)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6345..6361)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6354..6376)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6503..6958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6590..6609)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6800..6821
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6984..7088
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7089..7157,7158..7949)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7307..7326)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8120..8708
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8609..8628
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8862..8879
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8996..9017
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9032..9037,9038..9055,9056..9062)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9070..9086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9075..9097
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9094..9110
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9094..9110
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9129..9149
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9131..9149
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcag cagctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     1801 gagactagcc tcgagcggga tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     1861 ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc
     1921 ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct tagaacccca
     1981 gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac tggttttctt
     2041 tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg cggagggatc
     2101 tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg cacagctagt
     2161 tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt
     2221 ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc gctcggtggg
     2281 acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc
     2341 tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa
     2401 gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca
     2461 agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg gtgagatggg
     2521 ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga actcggtttg
     2581 tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc cgtacctttg
     2641 ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat aatgcagggt
     2701 ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg cagggttcgg
     2761 gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg gagggataag
     2821 tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag ctgaagctcc
     2881 ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt taggcacctt
     2941 ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt aaattgtccg
     3001 ctaaattctg gccgtttttg gcttttttgt tagacgaagc gctagcgcta ccggtttaat
     3061 taacctgaca atggagatgg agcaggagaa aatgacgatg aataaagagc ttagcccaga
     3121 cgctgccgca tattgctgtt cagcgtgcca tggtgacgaa acatggagct ataaccatcc
     3181 tatacgaggc agagcaaaga gcagaagctt gtctgcgtcc ccagccctcg ggtctactaa
     3241 ggaattccgc cgaactagaa gtctccacgg gccttgtcct gtgaccacat ttgggcccaa
     3301 ggcatgtgtt cttcagaatc cgcagacaat catgcacatt caggatccag cttcccagag
     3361 gctcacgtgg aataagagtc caaagtccgt tctcgtaatc aaaaaaatgc gggatgcgtc
     3421 actcctccaa ccgttcaaag agctttgcac acacttgatg gaagaaaata tgatcgttta
     3481 cgtcgaaaag aaagtactcg aggacccggc gatcgcttct gatgaaagtt ttggcgctgt
     3541 gaaaaaaaaa ttttgtacgt ttcgggaaga ttacgacgac atatccaatc agatcgactt
     3601 tataatctgt ttgggagggg acgggacgct tctgtatgca tcctcccttt ttcagggcag
     3661 cgttccacct gtcatggcat tccatctcgg ctctcttggt tttctcacgc cattctcttt
     3721 tgagaacttt caatctcagg taacgcaggt catagagggt aacgccgccg ttgttttgcg
     3781 ctcccgcctg aaagtcaggg ttgtaaagga actgcgaggg aagaaaacag ccgtccataa
     3841 cggtctcgga gaaaacggga gtcaagcagc cggcctggac atggatgtag gcaaacaggc
     3901 tatgcaatat caagtcctga acgaagttgt tatcgaccgc ggcccgagtt cctacctcag
     3961 taacgtagac gtatatctgg acggtcattt gattaccacg gtccaaggtg atggggttat
     4021 cgtgagcaca cccaccgggt ccacagccta tgcagccgct gcaggtgcgt caatgatcca
     4081 cccaaacgtg ccggccataa tgatcacacc gatttgtcct cactcactct ctttccgacc
     4141 gatagtagta ccggccggag tagagttgaa aattatgctg tcccctgaag cgcggaatac
     4201 cgcttgggtg tccttcgatg ggagaaaacg gcaggaaatc cggcacgggg attccataag
     4261 tatcaccact tcctgctatc ctctgccgag catttgtgtg cgcgacccgg tatcagattg
     4321 gtttgaatcc ctggctcaat gtcttcattg gaacgttcgc aagaagcagg ctcattttga
     4381 ggaggaagag gaagaagaag aggagggcgg aggcagcgcg gccgcaggcg actacaaaga
     4441 ccatgacggt gattataaag atcatgacat tgattacaag gatgacgatg acaagtaata
     4501 agaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     4561 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     4621 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     4681 cagagatcca ctttggccgc ggctcgaggg ggttggggtt gcgccttttc caaggcagcc
     4741 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     4801 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     4861 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     4921 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     4981 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     5041 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     5101 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     5161 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
     5221 gggatccatg gttcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     5281 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     5341 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     5401 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     5461 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     5521 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     5581 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taaggtacct ttaagaccaa
     5641 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     5701 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     5761 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5821 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5881 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     5941 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     6001 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6061 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6121 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     6181 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6241 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6301 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     6361 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     6421 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     6481 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6541 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6601 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6661 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6721 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6781 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6841 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6901 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     6961 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     7021 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     7081 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     7141 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     7201 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     7261 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7321 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7381 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7441 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7501 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7561 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7621 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7681 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7741 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7801 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7861 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7921 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7981 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     8041 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     8101 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     8161 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     8221 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     8281 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8341 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8401 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8461 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8521 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8581 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8641 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8701 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8761 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8821 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8881 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8941 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     9001 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     9061 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     9121 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaa
//
