LOCUS       sequence-32456-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Takagi J, Petre BM, Walz T, Springer TA
  TITLE     Global conformational rearrangements in integrin extracellular 
            domains in outside-in and inside-out signaling.
  JOURNAL   Cell. 2002 Sep 6. 110(5):599-11.
  PUBMED    12230977
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2002 
            Sep 6. 110(5):599-11."
COMMENT     Sequence Label: pEF1-alpha-IIb
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..20)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(30..71)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tcatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag agggttaggg
       61 ataggcttac cttcgaaggg ccctctagac tccccctctt catcatcttc ttccaggggt
      121 ggccggttcc gcttgaagaa gccgaccttc cacatggcca ggaccaggat ggtgagcagc
      181 agcaggccac ccagcacacc caccagcacc caccagattg gaatggccct ctcctccaag
      241 gcccggagca gctgtgtcca cacctgagct tcccctcggg gcaggctgag cgggggcacc
      301 gcatagggga gggaggacac gttgaaccat gcgtgcgact gcagcacaaa ctgatccaga
      361 ggcctctggt agaggctggg cagccacagg aaggccagca ccgtgaccat ggcccgctgc
      421 ccgcgcgcca tctcctgcag gtcacactgc accacagtac agggcgccga gtcgcagctt
      481 acgagaactg gatcctgaag cctcgagggc tgctcgggct ctggcaggaa gatctgtctg
      541 cgatcccgct tgtgatgggc cgggtgaatg ggggaggggc tggggatggg cagcccccag
      601 tccaccttga gagggttgac aggaggctgt gggaagcact gaaggccccc ctggggctgt
      661 atatccagga tgtagagcag gtcggagggc tgggactgtc ccggaaggtg gatgctgagg
      721 tgaagaccat tcacagtccc agggccattg ttgtggagct cataggtgtg ctccactttg
      781 ggtccccagc tgtccaagct gttctgctcc ctctcacctt cttctgctgc caccaccagg
      841 ga
//
