LOCUS       sequence-32457-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 hsa-mir-124
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(581..605)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             631..672
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(712..731)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    756..804
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 aagcaaactc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagt taagctatca acaagtttgt acaaaaaagc
      121 aggctcatat gatgctgtgg tcccttcctc cggcgttccc cacccccatc cctctccccg
      181 ctgtcagtgc gcacgcacac gcgccgcttt ttatttcttt ttcctggttt tcttattcca
      241 tcttctaccc acccctcttc ctttctttca cctttccttc cttccttcct cctttccttc
      301 ctcaggagaa aggcctctct ctccgtgttc acagcggacc ttgatttaaa tgtccataca
      361 attaaggcac gcggtgaatg ccaagaatgg ggctggctga gcaccgtggg tcggcgaggg
      421 cccgccaagg aaggagcgac cgaccgagcc aggcgccctc cgcagacctc cgcgcagcgg
      481 ccgcgggcgc gaggggaggg gtctggagct ccctccggct gcctgtcccg caccggagcc
      541 cgtggggtgg ggaggtgtgc agcctgtgac agacaagctt acccagcttt cttgtacaaa
      601 gtggttgatc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
      661 ctcgattcta cgcgtaccgg ttagtaatga gtttaaacgg gggaggctaa ctgaaacacg
      721 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
      781 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
      841 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc tttttcccac
      901 cccanccccc aa
//
