LOCUS       sequence-32459-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 hsa-mir-128
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    97..121
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(480..504)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             530..571
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(611..630)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    655..703
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gcgactcctg gctactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagttaag ctatcaacaa gtttgtacaa aaaagcaggc
      121 tcatatgcaa agccctagct gttttctgtg tagcttttat tattcttatg actcttgaca
      181 agtttgtagc ttcaccatat acatttaata ttttgcaata attggccttg ttcctgagct
      241 gttggattcg gggccgtagc actgtctgag aggtttacat ttctcacagt gaaccggtct
      301 ctttttcagc tgcttcctgg cttcttttta ctcaggtttc cactgctttt ttgctttttt
      361 taatgctgta tgaaggtgtt aacatttgtt tatatttttc attaattgta atacctttaa
      421 atcatgcatc atactcagaa atagggatta gaatttaagt gacatctttg gccaagctta
      481 cccagctttc ttgtacaaag tggttgatct agagggcccg cggttcgaag gtaagcctat
      541 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag tttaaacggg
      601 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
      661 taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg
      721 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
      781 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
      841 aacgtcgggg cggcaggccc tgccatagca gatctgcgca gctggggctc tagggggtat
      901 cccccacgcg ccctgtagcg gcgcataaa
//
