LOCUS       sequence-32460-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 hsa-mir-142
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    105..129
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(542..566)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             592..633
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(673..692)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    717..765
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ctttagcaga gctctctggc tactagagaa cccactgctt actggcttat cgaaattaat
       61 acgactcact atagggagac ccaagctggc tagttaagct atcaacaagt ttgtacaaaa
      121 aagcaggctc atatggggag gtagaggagg caagtctggc gccatgctga gtcaccgccc
      181 acaaggccca gggcgggccc tcggggggcc ctggcagggt tggggggatc ttaggaagcc
      241 acaaggaggg ctggggggct cttggagcag gagtcaggag gcctgggcag cctgaagagt
      301 acacgccgac ggacagacag acagtgcagt cacccataaa gtagaaagca ctactaacag
      361 cactggaggg tgtagtgttt cctactttat ggatgagtgt actgtgggct tcggagatca
      421 cgccactgct gccgcccgct gcccgccacc atcttcctcg gcgctcgggg acctcgtgtg
      481 acaggtgagc accttacggc ccctccctac cctgcccaga tgcctgaaag gcctcaagct
      541 tacccagctt tcttgtacaa agtggttgat ctagagggcc cgcggttcga aggtaagcct
      601 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg agtttaaacg
      661 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
      721 aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc
      781 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
      841 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
      901 ccaacgtcgg ggcggcaggc cctgccat
//
