LOCUS       sequence-32462-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 hsa-mir-192
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(525..549)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             575..616
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(656..675)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    700..748
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 agcaaactct ctggctacta gaagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagt taagctatca acaagtttgt acaaaaaagc
      121 aggctcatat ggtggaagtg cccactggtt ccagtggggc tgctgttatc tggggcgagg
      181 gccagtaccc acgaagaagg agaggcaggt gctggccagc agaccagcca ggactaccgt
      241 ggcgacgctc ccaggccaga tggtggcggg tagtggaggg ctgtcggtgg gctgccgaga
      301 ccgagtgcac agggctctga cctatgaatt gacagccagt gctctcgtct cccctctggc
      361 tgccaattcc ataggtcaca ggtatgttcg cctcaatgcc agccaccagg acctgcaggg
      421 ataggggagg gccgggggtg tccagcagtc agcagagatc ctgcgacccc agtgcagcac
      481 tcatggtccc acctccctct gtctcattcc ccgtgaataa gcttacccag ctttcttgta
      541 caaagtggtt gatctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
      601 cggtctcgat tctacgcgta ccggttagta atgagtttaa acgggggagg ctaactgaaa
      661 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
      721 aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
      781 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
      841 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
//
