LOCUS       sequence-32463-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 hsa-mir-205
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    104..128
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(561..585)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             611..652
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(692..711)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    736..784
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gctttagcga ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagctggct agttaagcta tcaacaagtt tgtacaaaaa
      121 agcaggctca tatgagcctc cttggaggat gtgatcctat gggaggggta ggagtattca
      181 ggtccttgac atctcccaaa tgtgtgattc cgggatgcca aaggcctttg gccaggtaat
      241 gcagtgtcta caggctgagg ttgacatgca tccccaccct ctgagaaaaa gatcctcaga
      301 caatccatgt gcttctcttg tccttcattc caccggagtc tgtctcatac ccaaccagat
      361 ttcagtggag tgaagttcag gaggcatgga gctgacaacc atgaggcctc ggcagccacc
      421 gccaccaccg ccgccgccac caccgtagca gcagcagcag cagcagcagc agcagcagca
      481 gcagcaagag taactctgac ttaggaatag agacagccag agagaaatgt gatcaatgaa
      541 agagacatct ggagaagctt acccagcttt cttgtacaaa gtggttgatc tagagggccc
      601 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
      661 ttagtaatga gtttaaacgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
      721 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
      781 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
      841 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagtttcggg
      901 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgcctaa
//
