LOCUS       sequence-324631-        9897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9897)
  AUTHORS   Jeanneteau F, Diaz J, Sokoloff P, Griffon N
  TITLE     Interactions of GIPC with dopamine D2, D3 but not D4 receptors 
            define a novel mode of regulation of G protein-coupled receptors.
  JOURNAL   Mol Biol Cell. 2004 Feb . 15(2):696-705.
  PUBMED    14617818
REFERENCE   2  (bases 1 to 9897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2004 Feb . 15(2):696-705."
COMMENT     Sequence Label: GFP-DRD4
FEATURES             Location/Qualifiers
     source          1..9897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      787..796
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(793..795,796..798,799..1509)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(793..795,796..798,799..1509)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(838..859)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1099..1118)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1446..1467
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(2085..2096)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4954..4970
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4955..4971
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5085..5102)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5091..5315
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5361..5789
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5448..5467)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5658..5679
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5798..5818)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5803..6132
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5983..6118
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6045..6064
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6181..7206
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6181..7206
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7336..7457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7373..7392)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7427..7446
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7506..7522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7506..7522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7519..7541)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7530..7546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7554..7560,7561..7578,7579..7584))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7599..7620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7737..7754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7908..8496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7988..8007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8667..9458,9459..9527))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9290..9309
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9528..9632)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9707..9726)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagactcg agggccgccc agcttccacc atgggcctcc gggcgctgat gctgtggttg
      721 ctggcggcga cggggctcgt gcgcgagtcc ctgcaaggag agttccaaag gaagctgtac
      781 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      841 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      901 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      961 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     1021 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1081 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     1141 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1201 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1261 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1321 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1381 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1441 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1501 ctgtacaagt ccggactcag atctcgaggg cgcgccatgg ggaaccgcag caccgcggac
     1561 gcggacgggc tgctggctgg gcgcgggccg gccgcggggg catctgcggg ggcatctgcg
     1621 gggctggctg ggcagggcgc ggcggcgctg gtggggggcg tgctgctcat cggcgcggtg
     1681 ctcgcgggga actcgctcgt gtgcgtgagc gtggccaccg agcgcgccct gcagacgccc
     1741 accaactcct tcatcgtgag cctggcggcc gccgacctcc tcctcgctct cctggtgctg
     1801 ccgctcttcg tctactccga ggtgagccgc gtccggccgc acgagcatcc tcacctgctc
     1861 ctcggttccc cgtccctgtc cctacggagg acccggcgcg acccggcccc tttctggtgc
     1921 ggagcttcca gctggggcgg cggcaggggc gctgcgcctt gtccctcggc gatacaccca
     1981 ccgccgccac ctcgcgacct tccacccgct gcgctgtctg tcccccgacc ctcgttcctc
     2041 ttctccttcc ccgtctgtct tggcgtctgt tatccaggag atgcccgtcc ttctatccag
     2101 ggaccccgga aacaggcgac tttgtcaagc ccagtcccct ccgtagctgg atttcacctc
     2161 cagggcagcc agctggacag acaggcagat gcaggctcag ccccctggct gccgtgggac
     2221 acacacacac acactgccac agccactgcc caccacacac acctagtgca gatgctggca
     2281 cacccccaga aggaggctca cagctcgcag gggagacctg ggctggacaa aacccagggg
     2341 aggggagggt gtgtggggac caggcccctg ctgagaaccc tggggggaag cctgaggggg
     2401 aattggggga tggagcccac actccacacc aggtctggcc ctcgagtggg tcggccttgg
     2461 tgccagcccc tctgcggcca gagaaaagca gcttagggct gagctggaga cgcggtgtcc
     2521 ccgactgtgg gggaggggga ctcgaggttt ccccttgatg gacacagtga atccaggcgg
     2581 ctggggcaga gaccagcagc acgggacacg cgtgacctgt gctcctttcg agccgcagac
     2641 gtcacagtga cgacgtttaa gctcctaatc tccccaaatc ggcgggaagg attagagggg
     2701 ctgcctgctc ctttgccctt ggagagagtc actccacgtg gagtcctacg ctgggcactg
     2761 ggcacggtct tcatattttt aattataatt tatcttcaag acaattatga tgtggatact
     2821 atcatcaccc ttgtcttaca gatgaagaaa cagaaatgaa ttaacgtgcc cgagtctccc
     2881 acagagaact tagccaacag agcagccagc ttcgcttcta ggcccagcgg ggaccctgcc
     2941 taccccaggc tggggagggg ctccctttga gcccgatgat atcaggcccg cccccaggga
     3001 attccccagg ccgttctcat gtttgtaaca aaagggagac caaggccaga agccgtgatt
     3061 cacgcctgta atcccaggac tttgggaggc aaaggcagga ggatagtttg agaccagcct
     3121 gggaaacata ccaggcctgg tctctacaaa aaattttaaa atatcagccg ggggtggtgg
     3181 ctgcggctgt tgtcccagct attcggggga gctgaggcgg cagaatcgct taaacctggg
     3241 aggcggaggt tgcagtgagt tgagatctca ccactgcact ccagcctggg caacaagagc
     3301 gaaactccgt ctcaaaaaac aaaaagaaaa acaaatcagc cgggggtggt ggcgcgcggc
     3361 tgtaatccca gctactctgg agactgaggt gggaggatcg cttgagctca ggaattccag
     3421 gctacagtga gccatgatgg agccacagca ctccagccgc ggtgacacag cgagacccta
     3481 actcaaaaca aagggagatc tgagtgggga aggggtgttt ccctgcccgg tcctctggcc
     3541 tctggctcac agccgggccc ccttctccgt attcagccct ggaactaccc ataagagtgg
     3601 gggggtcaca agggcccgcg gtggctggga aacctcaggg cctgtggtgt cgccgcgcag
     3661 gtccagggtg gcgcgtggct gctgagcccc cgcctgtgcg acgccctcat ggccatggac
     3721 gtcatgctgt gcaccgcctc catcttcaac ctgtgcgcca tcagcgtgga caggtgcgcc
     3781 gccctccccg cccgcgcccc ggcgcccccg cgccccgccc gccgccctca ccgcggcctg
     3841 tgcgctgtcc ggcgccccct cggcgctccc cgcaggttcg tggccgtggc cgtgccgctg
     3901 cgctacaacc ggcagggtgg gagccgccgg cagctgctgc tcatcggcgc cacgtggctg
     3961 ctgtccgcgg cggtggcggc gcccgtactg tgcggcctca acgacgtgcg cggccgcgac
     4021 cccgccgtgt gccgcctgga ggaccgcgac tacgtggtct actcgtccgt gtgctccttc
     4081 ttcctaccct gcccgctcat gctgctgctc tactgggcca cgttccgcgg cctgcagcgc
     4141 tgggaggtgg cacgtcgcgc caagctgcac ggccgcgcgc cccgccgacc cagcggccct
     4201 ggcccgcctt cccccacgcc acccgcgccc cgcctccccc aggacccctg cggccccgac
     4261 ygtgcgcccc ccgcgccccg ccyccccccc grccccsgcc csccyycccc cmcscccccc
     4321 gcgccccgcc tcccccagga cccctgcggc cccgactgtg cgccccccgc gcccggcctc
     4381 cccccggacc cctgcggctc caactgtgct ccccccgacg ccgtcagagc cgccgcgctc
     4441 ccaccccaga ctccaccgca gacccgcagg aggcggcgtg ccaagatcac cggccgggag
     4501 cgcaaggcca tgagggtcct gccggtggtg gtcggggcct tcctgctgtg ctggacgccc
     4561 tkcwkcgkgs kgcryrycam gmwsrcssks ygksmksscw rggccatgag ggtcctgccg
     4621 gtggtggtcg gggccttcct gctgtgctgg acgcccttct tcgtggtgca catcacgcag
     4681 gcgctgtgtc ctgcctgctc cgtgcccccg cggctggtca gcgccgtcac ctggctgggc
     4741 tacgtcaaca gcgccctcaa ccccgtcatc tacactgtct tcaacgccga gttccgcaac
     4801 gtcttccgca aggccctgcg tgcctgctgc tgagccgggc acccccggac gccccccggc
     4861 ctgatggcca ggcctcaggg accaaggaga tggggagggc gcttttgtac gttaattaaa
     4921 caaattcctt cccaaaaaaa aaaaaaaaaa anctcgaggt cgacggtatc gataagcttg
     4981 gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg
     5041 gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     5101 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     5161 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     5221 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     5281 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     5341 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5401 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5461 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5521 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     5581 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5641 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5701 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5761 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     5821 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5881 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     5941 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     6001 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6061 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6121 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     6181 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     6241 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     6301 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     6361 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     6421 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     6481 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     6541 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     6601 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     6661 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     6721 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     6781 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     6841 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     6901 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     6961 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     7021 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     7081 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     7141 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     7201 gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     7261 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     7321 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7381 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7441 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     7501 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7561 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7621 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7681 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7741 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7801 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7861 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7921 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7981 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8041 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8101 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8161 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8221 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8281 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8341 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8401 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8461 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8521 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8581 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8641 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8701 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8761 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8821 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8881 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8941 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9001 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9061 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9121 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9181 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9241 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9301 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9361 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9421 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9481 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9541 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9601 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     9661 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     9721 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     9781 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     9841 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
