LOCUS       sequence-324633-        7816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Kerns JA, Emerman M, Malik HS
  TITLE     Positive selection and increased antiviral activity associated with 
            the PARP-containing isoform of human zinc-finger antiviral protein.
  JOURNAL   PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1371/journal.pgen.0040021.
  PUBMED    18225958
REFERENCE   2  (bases 1 to 7816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2008-01"; volume: "4(1):e21. doi"; pages: " 
            10.1371/journal.pgen.0040021"
COMMENT     Sequence Label: pcDNA4 huZAP(L)
FEATURES             Location/Qualifiers
     source          1..7816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             724..750
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3505..3534
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3550..3567
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3590..3607)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3596..3820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3866..4294
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3953..3972)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4163..4184
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4303..4323)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4308..4637
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4488..4623
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4550..4569
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4685..4732
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4751..5125
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5255..5376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5292..5311)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5346..5365
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5425..5441)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5425..5441)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5438..5460)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5449..5465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5473..5479,5480..5497,5498..5503))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5518..5539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5656..5673)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5827..6415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5907..5926)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6586..7377,7378..7446))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7209..7228
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7447..7551)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7626..7645)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga attcgccacc
      721 atgtatccat acgatgttcc agattacgct gcggacccgg aggtgtgctg cttcatcacc
      781 aaaatcctgt gcgcccacgg gggccgcatg gccctggacg cgctgctcca ggagatcgcg
      841 ctgtctgagc cgcagctctg tgaggtgctg caggtggccg ggcccgaccg ctttgtggtg
      901 ttggagaccg gcggcgaggc cgggatcacc cgatcggtgg tggccaccac tcgagcccgg
      961 gtctgccgtc gcaagtactg ccagagaccc tgcgataacc tgcatctctg caaactcaac
     1021 ttgctgggcc ggtgcaacta ttcgcagtcc gagcggaatt tatgcaaata ttctcatgag
     1081 gttctctcag aagagaactt caaagtcctg aaaaatcacg aactctctgg actgaacaaa
     1141 gaggaattag cagtgctcct cctccaaagt gatccttttt ttatgcccga gatatgcaaa
     1201 agttataagg gagagggtcg gcagcagatt tgtaaccagc agccaccgtg ttcaagactc
     1261 cacatctgtg accacttcac ccgagggaac tgtcgttttc ccaactgcct ccggtcccat
     1321 aacctgatgg acagaaaggt gctggccatc acgagggagc acgggctgaa ccccgacgtg
     1381 gtccagaaca tccaggacat ctgcaacagc aagcacatgc agaagaatcc cccagggccc
     1441 agagctcctt cttcacatcg tagaaacatg gcatataggg ctagaagcaa gagtagagat
     1501 cggttctttc agggcagcca agaatttctt gcgtctgctt cagcgtctgc tgagaggtcc
     1561 tgcacaccta gtccagatca gatcagccac agggcttccc tggaggacgc gcctgtggac
     1621 gatctcaccc gcaagttcac gtatctgggg agtcaggatc gcgctcggcc tccctcaggc
     1681 tcgtccaagg ctactgatct tggaggaaca agtcaggccg ggacaagcca gaggttttta
     1741 gagaacggca gtcaagagga cctcttgcat ggaaatccag gcagcactta ccttgcttcc
     1801 aattcaacat cagcccccaa ctggaagagc ctcacatcct ggacgaatga ccaaggcgcc
     1861 aggagaaaga ctgtgttttc tcccacgcta cctgccgccc gctcttcttt tggctctctg
     1921 caaacacctg aagctgtgac caccagaaag ggcacaggct tgctttcctc agactacagg
     1981 atcatcaatg gcaaaagtgg aactcaggac atccagcctg gccctctttt taataataat
     2041 gctgatggag tggccacaga tataacttct accagatcct taaattacaa aagcactagc
     2101 agcggtcaca gagaaatatc atcacctagg attcaggatg ctggacctgc ttcccgagat
     2161 gtccaggcca ctggcagaat cgcagatgat gctgacccaa gagtagcact tgttaacgat
     2221 tctttatctg atgtcacaag taccacatct tctagggtgg atgatcatga ctcagaggaa
     2281 atttgtcttg accatctgtg taagggttgt ccgcttaatg gtagctgcag caaagtccac
     2341 ttccatctgc cttaccggtg gcagatgctt attggtaaaa cctggacgga ctttgagcac
     2401 atggagacga tcgagaaagg ctactgtaac cccggaatcc acctctgttc tgtaggaagt
     2461 tatacaatca attttcgggt aatgagttgt gattcctttc ccatccgacg cctctccact
     2521 ccttcttctg tcaccaagcc agccaattct gtcttcacca ccaaatggat ttggtattgg
     2581 aagaatgaat ctggcacatg gattcagtat ggagaagaga aagacaaacg gaaaaattca
     2641 aacgtcgact cttcatacct ggagtctctc tatcaatcct gtccgagggg agttgtgcca
     2701 tttcaggcgg gctcacggaa ctatgagctg agtttccaag ggatgattca gacaaacata
     2761 gcttccaaaa ctcaaaagga tgtcatcaga agaccaacat ttgtgcctca gtggtatgtg
     2821 cagcagatga agagagggcc agaccatgag ccagcaaaga cctcgtcagt gtctttaact
     2881 gcgacctttc gtcctcagga ggacttttgc ttcctatcct caaagaaata taagttgtca
     2941 gagatccatc acctacatcc agaatatgtc agagtaagtg agcattttaa agcttccatg
     3001 aaaaatttca agattgaaaa gataaagaag atcgagaact cagagctcct ggataaattt
     3061 acatggaaga aatcgcagat gaaggaagaa ggaaaactcc tattttatgc gacaagccgt
     3121 gcctatgtgg aatctatctg ttcgaataat tttgacagtt tcctacatga aactcatgaa
     3181 aacaaatacg gaaaaggaat ttactttgca aaagatgcca tctattccca caaaaattgc
     3241 ccgtatgatg ccaaaaacgt cgttatgttt gtagcccaag ttctggttgg aaagtttatt
     3301 gaaggaaata taacgtacac gagccctcct ccacagtttg acagctgtgt ggataccaga
     3361 tcgaatccct ccgtttttgt catctttcag aaagatcagg tttacccaca atatgtgatt
     3421 gaatatactg aagacaaagc ctgcgtgatt agttaggata tccagcacag tggcggccgc
     3481 tcgagtctag agggcccgcg gttcgaacaa aaactcatct cagaagagga tctgaatatg
     3541 cataccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
     3601 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     3661 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     3721 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     3781 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     3841 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3901 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3961 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4021 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4081 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4141 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4201 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4261 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     4321 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4381 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4441 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4501 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4561 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4621 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     4681 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     4741 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     4801 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     4861 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     4921 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     4981 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     5041 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     5101 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     5161 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     5221 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     5281 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5341 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     5401 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     5461 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     5521 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     5581 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     5641 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5701 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5761 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5821 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5881 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5941 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6001 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6061 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6121 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6181 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6241 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     6301 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6361 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6541 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6721 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7441 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7501 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7561 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     7621 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     7681 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     7741 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     7801 gccagatata cgcgtt
//
