LOCUS       sequence-32465-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 hsa-mir-220a
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(546..570)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             596..637
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(677..696)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    721..769
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tagcaaactc tctggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagt taagctatca acaagtttgt acaaaaaagc
      121 aggctcatat gcacaggcag gtggtgactc tgctcatggt ggctgacacc aggtggttga
      181 ggtccccgca ggtggaggtg gtcagcttga gggtgcggaa gcagatgtca tagagtgccc
      241 cattgttgat gcagtaggtc tcattcgtat tctccaccag ctggtgcaca gacagtgtgg
      301 cattgtaggg ctccacaccg tatctgacac tttgggcgag ggcaccatgc tgaaggtgtt
      361 catgatgcgg tctgggaact cctcacggat cttactgatg agcagtgtgc ccatcccgga
      421 ccccgtgcca ccccccagca agtgcgtcag ctggaagcct tgaaggcagt cgcagttcta
      481 ggcctccttc tggaccacgt ccaggacagt gcctaccagc tctgtgccct ccgtgtagta
      541 agcttaccca gctttcttgt acaaagtggt tgatctagag ggcccgcggt tcgaaggtaa
      601 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagttta
      661 aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
      721 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
      781 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
      841 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggttgaa
//
