LOCUS       sequence-32466-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 hsa-mir-483
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..35
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(442..466)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(495..514)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(496..514)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(559..762)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(588..608)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cctctagatc accactttgt acaagaaagc tgggtaagct tacaatggga gtggtttgga
       61 aaatgtgtgg gatgtgagcc cagttcaggg gcccccacag gagggactgc tgtccctgag
      121 cttggactct ggcctggcac cacctaggag gctggagggg cccacaccac ccctggagct
      181 gctgagacag ggagacaagg gcaggagagg agaagacggg aggagaggag tgaggaggcg
      241 tgatggaacc actcccttct ttcctcccgt cttccccctc tcccccacag caatgctcag
      301 ctggaagggg aaggtcgctg ggggctccac cttggggtgc caaagggcag gtgccatcag
      361 ccggaggagg gagcaggagc cccatcacgg gggtccccca agtgggggct tggccctccc
      421 ctcctctcag gaaagcatat gagcctgctt ttttgtacaa acttgttgat agcttaacta
      481 gccagcttgg gtctccctat agtgagtcgt attaatttcg ataagccagt aagcagtggg
      541 ttctctagtt agccagagag ctctgcttat atagacctcc caccgtacac gcctaccgcc
      601 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
      661 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
      721 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
      781 taatacgtag atgtactgcc aagta
//
