LOCUS       sequence-32468-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 hsa-mir-888
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    99..123
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(467..491)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             517..558
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(598..617)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    642..690
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tagcgactct ctggctactg agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagtta agctatcaac aagtttgtac aaaaaagcag
      121 gctcatatgc ttctggtcct ggcaatcatc cagataaaca tagagtaggt gagagattcc
      181 tcacttaggg aggcatatac cttggagggc agtgggctag acatgatcca ggactgatga
      241 ataaataagt tcagggttca gacatcatgc tgtgctcaga ggagtgatgg aagctacagg
      301 tggagaacac gcaagatgcg acagcacctc cacaattagc catgttgtgg gcagtgctct
      361 actcaaaaag ctgtcagtca cttagattac atgtgactga cacctctttg ggtgaaggaa
      421 ggctccccaa gtacactttt gtggtggtcc tcagactggt aagcttaccc agctttcttg
      481 tacaaagtgg ttgatctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
      541 ctcggtctcg attctacgcg taccggttag taatgagttt aaacggggga ggctaactga
      601 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
      661 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
      721 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
      781 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
      841 caggccctgc catagcagat ctgcgcacct ggggctctag ggggtatccc cacgcgccct
      901 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
//
