LOCUS       sequence-32469-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 hsa-mir-9-1
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    104..128
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(443..467)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             493..534
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(574..593)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    618..666
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ctttagcaaa ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagctggct agttaagcta tcaacaagtt tgtacaaaaa
      121 agcaggctca tatgcgtgtc ccttccctcc tactcccgct gacgggcgac cgggaagctg
      181 cggaggtgct ggcggcggcg gcggccggga ggctgcgtgg aagaggcggc gacagcagcc
      241 aggaggcggg gttggttgtt atctttggtt atctagctgt atgagtggtg tggagtcttc
      301 ataaagctag ataaccgaaa gtaaaaataa ccccatacac tgcgcagagg ggcccgggga
      361 aagccggcct cacgggcggg agggagaggc ttgagcgagg ggctagagcc gcgaggactg
      421 accagcacca gaggataagc ttacccagct ttcttgtaca aagtggttga tctagagggc
      481 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
      541 ggttagtaat gagtttaaac gggggaggct aactgaaaca cggaaggaga caataccgga
      601 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt
      661 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
      721 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
      781 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcagatctgc
      841 gcagctgggg ctctaggggg tatccccacg cgcctgtacg cgcataaccg cgggtgtggt
      901 ncccactgac ct
//
