LOCUS       sequence-32470-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 hsa-mir-193b
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(538..562)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             588..629
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(669..688)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    713..761
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 actctctggc tactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagcaggctc
      121 atatggaggg aacacccagt aacgattcct tcagtgatcc cggttctcca aactcttgcc
      181 tcaaaggcgg cctgccccgg tgctgtcagc cgtgaatggg gactcacttc ttgggaaaag
      241 aggcttttgg aggctgtggt ctcagaatcg gggttttgag ggcgagatga gtttatgttt
      301 tatccaactg gccctcaaag tcccgctttt ggggtcattc tggacagcga gggactcagg
      361 ccccggcagt ctcgggaggg tgagggcaga gagctaccac ccccagggag cgcgtccaaa
      421 acccggctgg cgggggggcg gggaaagagg gggaggtttg ctactttcca taaaactaaa
      481 aggtcccttc gagggcggta ttcttataag tgtgtgttct cagtttgccg aaagcttacc
      541 cagctttctt gtacaaagtg gttgatctag agggcccgcg gttcgaaggt aagcctatcc
      601 ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagtt taaacggggg
      661 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
      721 aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
      781 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
      841 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcanccaa
      901 cgtc
//
