LOCUS       sequence-32472-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 hsa-mir-499
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(450..474)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             500..541
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(581..600)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    625..673
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 catagcaact ctctggctac tagagaacca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagt taagctatca acaagtttgt acaaaaaagc
      121 aggctcatat gggagacaga ccctccctct tggcagcctc cagccccgtc cttcaccccc
      181 tgccctgtcc cctgtgcctt gggcgggcgg ctgttaagac ttgcagtgat gtttaactcc
      241 tctccacgtg aacatcacag caagtctgtg ctgcttcccg tccctacgct gcctgggcag
      301 ggtttgtggg gaaggggtgg gagccctggg gctctgggag ggacggggat ttggcaggga
      361 agtgaagact ttggtggtgg tgatgggaga cagcgaaggg tctcccatca ccagaatgag
      421 agtgaaagag caaaagtgca gggaagctta cccagctttc ttgtacaaag tggttgatct
      481 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
      541 gcgtaccggt tagtaatgag tttaaacggg ggaggctaac tgaaacacgg aaggagacaa
      601 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt
      661 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
      721 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
      781 agttcgggtg aaggcccagg gctcgca
//
