LOCUS       sequence-324728-        5992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for the synthesis of zebrafish npas4l mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Mattonet K, Riemslagh FW, Guenther S, Prummel KD, Kesavan G, Hans S,
            Ebersberger I, Brand M, Burger A, Reischauer S, Mosimann C, Stainier
            DYR
  TITLE     Endothelial versus pronephron fate decision is modulated by the 
            transcription factors Cloche/Npas4l, Tal1, and Lmo2.
  JOURNAL   Sci Adv. 2022 Sep 2;8(35):eabn2082. doi: 10.1126/sciadv.abn2082. 
            Epub 2022 Aug 31.
  PUBMED    36044573
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-09-2"; volume: "8(35):eabn2082. doi"; pages: " 
            10.1126/sciadv.abn2082. Epub 2022 Aug 31"
COMMENT     Sequence Label: pCS2-npas4l
FEATURES             Location/Qualifiers
     source          1..5992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    2044..2178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2068..2087)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2122..2141
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2291..2311)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2291..2309)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2330..2346)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2330..2346)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2343..2365)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2354..2370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2378..2384,2385..2402,2403..2408))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2423..2444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2561..2578)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2732..3320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2812..2831)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3491..4282,4283..4351))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4114..4133
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4352..4456)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4482..4937)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4619..4640)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4831..4850
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5008..5992
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atccaccatg agctgcggag actcgggcat caggaggatc
      121 ctccgcgcgt ccaagcgctt caggtccacc aaaggagcat ctaaggcgcg gcgtgatcag
      181 atgaacagtg agatacggaa cctgcgagcg ctgctgccca tcagcccaga gcacagactc
      241 tcatacctgc acagcatgag catcacctgc acctacatca ggaagagtgt ggagctgaga
      301 ggtgtgtgtg aggaatccac agttttttct gccgtgaatg gatgtgtccc gcaggactgt
      361 gctctacagg actgtgctct acaggagtgt gttctgcagg agtgtgttct gcaggagtgt
      421 gttctgcagg agtgtgttct gcaggctctg ccggggttta tcgttgcctt taccacagat
      481 ggaaaactgc tgtatgtgtc tgagaacgta cacgagtatc tgggtctgtc catggtggac
      541 gttctccaga gcgactcttt cttcgatatg ctggaccgca gtgatgtgga ggcggtgagg
      601 agtgtgttag ctgacgccag tccttcagga gagcggtggg ttgtgtgtcg tatgctggtg
      661 tctaaagcga tgcgtctgcg gagctcctgc tgccccctgc tggtgcggat ccgtctgcgg
      721 gacggagtgt gtgtgtcgct ctgcagaccc accgctgacc ggctgccggc gcgcaacgct
      781 gacttccaca cacaccacag cgcagacatg aggctcgcca gcgcatcctc cagtgtgttg
      841 ttccacctgg gcttctcagc ggacgagctg attggtcgat cctggtacga gctcctgcac
      901 cccgacgacc tccgacacgc tgctgaccgc cacgccgcaa tccttgcagc cgccacagca
      961 gacgcagaga tgctgatccg ggttcagtgt aaggatctgt cctgggtgtg gatgtacaca
     1021 cacgcatcag caacagcaga gcgggacgcg atcagctgct ccaactacat gatcagtgaa
     1081 gcagaggcag tgtatctgca gcagagactc tcctcctcct cctcctcctc ctcctcctct
     1141 gcctctcctc agtgtagctc cgtctcggac agcagtgatg cccacagcgc acacacactc
     1201 ttctgcacgc cgccctacag ccccacttcc tctcagtgct ctgacttcct gtctgaaggt
     1261 tatggctctc tggaggcgct ggtggattct gccttctgct ctccgccgta cccgccgctg
     1321 ttccccaacc catgctgcac ccccactgtc tgcccaccgg acactgccct ggaccccgct
     1381 cggctctgcc cgccagattc tgccctggtc cccgctgcgc tctgtccgcc ggacgctgcc
     1441 ctggacccca ctcagctctg cccgccggga gctgccctgg accccactct gctctgcccg
     1501 ccgggagctg ccctggaccc cactcagctc tgcctgccgg acggtgcctt ggtccctgct
     1561 gtgttctgcc cgccgaacgg tgccctggtc cctgcccggc tctgcccgtc agatgctgcc
     1621 ctggtccccg ctgcactcgg tcggccggac gctgccctgg tgcctgtgtg tcggccactg
     1681 caggtgtgtg agtgccctct ggatggcgct gtgccgctgg aggacctcag catgttcccg
     1741 ctgccgcagg gcggaggctc tcgtctgatg ccgcctgaag catcacccac tacccacaca
     1801 cacttctcgt atgatgcagc acagcaggcg gggatcggca cgttggcaat gcagatacac
     1861 acactcatct gcagcttcga tgcgtacagc gccgcacgac acacacactc tcaccacacc
     1921 tcctgctggg caccagagcc actgctggac gagggcatca tcgacagcat cctcagagag
     1981 ctggacagca cacacactga tgcacacaca ctgatctaga actatagtga gtcgtattac
     2041 gtagatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     2101 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     2161 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     2221 gaggtgtggg aggtttttta attcgcggcc gcggcgccaa tgcattgggc ccggtaccca
     2281 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     2341 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc gggagcataa
     2401 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2461 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2521 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2581 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2641 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2701 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2761 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2821 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2881 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2941 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3001 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3061 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3121 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     3181 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3241 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3301 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3361 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3421 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3481 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3541 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     3601 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3661 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3721 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3781 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     3841 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     3901 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3961 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4021 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4081 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4141 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4201 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4261 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4321 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4381 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4441 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     4501 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     4561 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     4621 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4681 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4741 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4801 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4861 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4921 gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc
     4981 ggtgcgggcc tcttcgctat tacgccagtc gaccgccaat tcaatatggc gtatatggac
     5041 tcatgccaat tcaatatggt ggatctggac ctgtgccaat tcaatatggc gtatatggac
     5101 tcgtgccaat tcaatatggt ggatctggac cccagccaat tcaatatggc ggacttggca
     5161 ccatgccaat tcaatatggc ggacctggca ctgtgccaac tggggagggg tctacttggc
     5221 acggtgccaa gtttgaggag gggtcttggc cctgtgccaa gtccgccata ttgaattggc
     5281 atggtgccaa taatggcggc catattggct atatgccagg atcaatatat aggcaatatc
     5341 caatatggcc ctatgccaat atggctattg gccaggttca atactatgta ttggccctat
     5401 gccatatagt attccatata tgggttttcc tattgacgta gatagcccct cccaatgggc
     5461 ggtcccatat accatatatg gggcttccta ataccgccca tagccactcc cccattgacg
     5521 tcaatggtct ctatatatgg tctttcctat tgacgtcata tgggcggtcc tattgacgta
     5581 tatggcgcct cccccattga cgtcaattac ggtaaatggc ccgcctggct caatgcccat
     5641 tgacgtcaat aggaccaccc accattgacg tcaatgggat ggctcattgc ccattcatat
     5701 ccgttctcac gccccctatt gacgtcaatg acggtaaatg gcccacttgg cagtacatca
     5761 atatctatta atagtaactt ggcaagtaca ttactattgg aagtacgcca gggtacattg
     5821 gcagtactcc cattgacgtc aatggcggta aatggcccgc gatggctgcc aagtacatcc
     5881 ccattgacgt caatggggag gggcaatgac gcaaatgggc gttccattga cgtaaatggg
     5941 cggtaggcgt gcctaatggg aggtctatat aagcaatgct cgtttaggga ac
//
