LOCUS       sequence-324729-        5390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for the synthesis of zebrafish etsrp mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Mattonet K, Riemslagh FW, Guenther S, Prummel KD, Kesavan G, Hans S,
            Ebersberger I, Brand M, Burger A, Reischauer S, Mosimann C, Stainier
            DYR
  TITLE     Endothelial versus pronephron fate decision is modulated by the 
            transcription factors Cloche/Npas4l, Tal1, and Lmo2.
  JOURNAL   Sci Adv. 2022 Sep 2;8(35):eabn2082. doi: 10.1126/sciadv.abn2082. 
            Epub 2022 Aug 31.
  PUBMED    36044573
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-09-2"; volume: "8(35):eabn2082. doi"; pages: " 
            10.1126/sciadv.abn2082. Epub 2022 Aug 31"
COMMENT     Sequence Label: pCS2z-etsrp
FEATURES             Location/Qualifiers
     source          1..5390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1689..1709)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1689..1707)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1728..1744)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1728..1744)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1741..1763)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1752..1768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1776..1782,1783..1800,1801..1806))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1821..1842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1959..1976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2130..2718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2210..2229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2889..3680,3681..3749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3512..3531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3750..3854)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3880..4335)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4017..4038)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4229..4248
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4406..5390
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg ccaccatgga aatgtaccaa
      121 tctggatttt acacagaaga cttcagaact caggaggttc ctgctggttt cgacttcagt
      181 tcatatgact gcagtggtga agacctgtcc tttttattag acagcaaagg acccgtccaa
      241 cagcagtatg ccgaaaacta ctccgagcct cagaaggagc tgttgcacaa aggtcatgtt
      301 ctcacagtcg actctgggct gttcaatctg gactctttcc ctgagttcag caactgggca
      361 gcatacacta atattccaga aggaatggtt gcagacagac agcaggttgg ctttcaggaa
      421 tcaactcaaa cctaccagaa cctcgtccct ctctgcactc cagcacaaag cagtacattc
      481 agtcccacaa tggacaccag cagccattac caacctggga aaggcccaag tcacagagga
      541 gcatccggga ccgccagcct tgaccatttg ggtgaatcag acaggacata tggtttgtat
      601 gaagcagagc agcaaagcag gtcttcatat tggtccgact acccctcacc cggttactgc
      661 agctccatgc cgcttagcca gcctgcatcc tcctcatccc ctccggtcag tcagtccgct
      721 gagcacttct gcccccgcgt ggtcaaaaga cgcagcgctc ctcctcaaag atcagacaga
      781 gagggcgaga tcacgccaat gtccgcctac ccaggatctg gacccatcca gctctggcag
      841 tttctgctag aactcctgct ggattctgct tgccacactt ttataagttg gactggtgat
      901 ggctgggagt ttaaaatgtc agatcccgct gaggtggcga agcggtgggg ccagtgtaaa
      961 aacaagccta aaatgaacta tgagaagctc agtcgtggcc tgcgttacta ctaccacaaa
     1021 aacatcattc acaaaacggc gggaaagcgc tacgtctacc gctttgtctg tgacgtgcag
     1081 ggcatgcttg gaaaaactgc acatgaagtc ttggcaagtc taaacatctc cccgaatgca
     1141 gcatctccac agtctgtagc aaacacaagc cgctcagagg aaaccacaga gtcctggaca
     1201 cattagctcg agcctctagt aattaagtct cagccaccgt taactgaaca tgtcaaaacc
     1261 tgtggagact gttgagattt gatgttctga aaagataaag cctataaata aaatgttgcc
     1321 caaatttcct gcctgatgtt tttctttgtc tttgctacat ggctttgctg ctcggatcgg
     1381 ctcactctgt gtatgccacg ttcactttgt actctccttc tcacggtagg tttattattt
     1441 ttagatgtgc agttagtttc tgtgaaataa cacaccacac actgatattg tctgtgcatt
     1501 gacttggtga gtgcacattg tttttgatct tgacatattt atatttgatt gatcaggtga
     1561 actgtgtgaa tctaaagtgc tccatacaga tgttctgcat tgaaaatatt ctcattttat
     1621 tagtggaagt gagtgtatgc cagcggccgc ggcgccaatg cattgggccc ggtacccagc
     1681 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     1741 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg gagcataaag
     1801 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1861 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1921 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1981 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2041 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2101 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2161 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2221 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2281 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2341 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2401 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2461 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2521 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2581 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2641 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2701 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2761 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2821 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2881 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2941 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3001 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3061 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3121 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3181 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3241 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3301 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3361 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3421 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3481 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3541 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3601 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3661 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3721 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3841 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     3901 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     3961 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     4021 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     4081 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     4141 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     4201 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     4261 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     4321 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     4381 tgcgggcctc ttcgctatta cgccagtcga ccgccaattc aatatggcgt atatggactc
     4441 atgccaattc aatatggtgg atctggacct gtgccaattc aatatggcgt atatggactc
     4501 gtgccaattc aatatggtgg atctggaccc cagccaattc aatatggcgg acttggcacc
     4561 atgccaattc aatatggcgg acctggcact gtgccaactg gggaggggtc tacttggcac
     4621 ggtgccaagt ttgaggaggg gtcttggccc tgtgccaagt ccgccatatt gaattggcat
     4681 ggtgccaata atggcggcca tattggctat atgccaggat caatatatag gcaatatcca
     4741 atatggccct atgccaatat ggctattggc caggttcaat actatgtatt ggccctatgc
     4801 catatagtat tccatatatg ggttttccta ttgacgtaga tagcccctcc caatgggcgg
     4861 tcccatatac catatatggg gcttcctaat accgcccata gccactcccc cattgacgtc
     4921 aatggtctct atatatggtc tttcctattg acgtcatatg ggcggtccta ttgacgtata
     4981 tggcgcctcc cccattgacg tcaattacgg taaatggccc gcctggctca atgcccattg
     5041 acgtcaatag gaccacccac cattgacgtc aatgggatgg ctcattgccc attcatatcc
     5101 gttctcacgc cccctattga cgtcaatgac ggtaaatggc ccacttggca gtacatcaat
     5161 atctattaat agtaacttgg caagtacatt actattggaa gtacgccagg gtacattggc
     5221 agtactccca ttgacgtcaa tggcggtaaa tggcccgcga tggctgccaa gtacatcccc
     5281 attgacgtca atggggaggg gcaatgacgc aaatgggcgt tccattgacg taaatgggcg
     5341 gtaggcgtgc ctaatgggag gtctatataa gcaatgctcg tttagggaac
//
