LOCUS       sequence-32473-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 cand141
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(586..610)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             636..677
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(717..736)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    761..809
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 caaagcaact cttggctact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagt taagctatca acaagtttgt acaaaaaagc
      121 aggctcatat gcggctctgc ttttctgaga tggggcagct cccttctccc ttggcctcag
      181 gcctaggcgg gcgaggctgc tcccaccagc agggggcgct ttgactcttc cgtggagccc
      241 agctctgcgg ccgccacccc accccctgca cctgcctggc tgcacagacc tgctcctgaa
      301 tggcccctct gtgtggctgc tgctggctca gacccctgcc caccttgggc gggtgggtct
      361 gggtcttggc tggcacgcac acccaggggc ctgaaatcat ggaaaattcg agaatttccc
      421 atcttctcct ccaggttcag tcctgctcaa atgcagagcc gctcaaagct gccagctggc
      481 tggcctcccc tcccccgcag ccggcagccc cttggccctg ctcctcagtg acctggcccc
      541 tacccctgcc tagccctcca aatgacccag cctccagtca agcttaccca gctttcttgt
      601 acaaagtggt tgatctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc
      661 tcggtctcga ttctacgcgt accggttagt aatgagttta aacgggggag gctaactgaa
      721 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
      781 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
      841 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
      901 cccaccccac cccccaattt cgggtgaaag gccagggctc gca
//
