LOCUS       sequence-324730-        5264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for the synthesis of zebrafish tal1 mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Mattonet K, Riemslagh FW, Guenther S, Prummel KD, Kesavan G, Hans S,
            Ebersberger I, Brand M, Burger A, Reischauer S, Mosimann C, Stainier
            DYR
  TITLE     Endothelial versus pronephron fate decision is modulated by the 
            transcription factors Cloche/Npas4l, Tal1, and Lmo2.
  JOURNAL   Sci Adv. 2022 Sep 2;8(35):eabn2082. doi: 10.1126/sciadv.abn2082. 
            Epub 2022 Aug 31.
  PUBMED    36044573
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-09-2"; volume: "8(35):eabn2082. doi"; pages: " 
            10.1126/sciadv.abn2082. Epub 2022 Aug 31"
COMMENT     Sequence Label: pCS2z-tal1
FEATURES             Location/Qualifiers
     source          1..5264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1563..1583)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1563..1581)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1602..1618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1602..1618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1615..1637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1626..1642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1650..1656,1657..1674,1675..1680))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1695..1716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1833..1850)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2004..2592)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2084..2103)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2763..3554,3555..3623))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3386..3405
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3624..3728)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3754..4209)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3891..3912)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4103..4122
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4280..5264
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg ccaccatgat ggaaaaactg
      121 aaatccgagc aatttccgct gagcccgagt gcggagggct gcgcttctcc cccacgcggg
      181 gatggagacg cgcgcgggaa acaggaaggc acgacggcgg agacaggaga gcatcgcctc
      241 cccgaggagc tcaatggtgt tgccaaggaa acggctcacc acgccaccga gctaaaaaag
      301 gaggtggcgg tgatcgagct gtccaggaga ggagggagcg cggatataaa aggcagggag
      361 ctcaaggcag aactcagcca taaggtgcag accactgagc tgtgcagacc cccgattcca
      421 ttgccactgc ctcccagaga cccgctgagc gacactcgaa tggtgcagtt gagtccgccc
      481 gctttccctc tcccggcacg agcgatgctc tacagcaaca tgacgacgcc gctcgccact
      541 attaacagtg gttttgctgg agatgcggaa cagtatggga tgtatcctag caatcgagtc
      601 aagcgcagac ctgcacctta tgaggttgaa atcaacgatg gttcgcagcc caaaattgtg
      661 cgacggattt tcacgaacag tcgcgagcgc tggcgacagc agaatgtgaa cggtgccttc
      721 gcagagctgc gcaagctcat cccaacacac cctcctgaca agaaactcag caagaacgag
      781 atcctgcgtc tggccatgaa gtacatcaac tttctggcta agctcctcaa cgaccaggac
      841 gacatggtcg gcggtgaagc tcccgcccgg gccaatcggg acagcagaga tgcaactcta
      901 gtgcgggacg acctccttca ggagatgcta agccccaact ccagctgcgg gagtctgctg
      961 gatggggatg ccagcccaga gagctttact gaggaccagg actcatcagt ggagtccagg
     1021 ccctctgcaa gaggactaca ccattccagc ctaccgctgg acggaaatgc ccagcggtga
     1081 ctcgagcctc tagtaattaa gtctcagcca ccgttaactg aacatgtcaa aacctgtgga
     1141 gactgttgag atttgatgtt ctgaaaagat aaagcctata aataaaatgt tgcccaaatt
     1201 tcctgcctga tgtttttctt tgtctttgct acatggcttt gctgctcgga tcggctcact
     1261 ctgtgtatgc cacgttcact ttgtactctc cttctcacgg taggtttatt atttttagat
     1321 gtgcagttag tttctgtgaa ataacacacc acacactgat attgtctgtg cattgacttg
     1381 gtgagtgcac attgtttttg atcttgacat atttatattt gattgatcag gtgaactgtg
     1441 tgaatctaaa gtgctccata cagatgttct gcattgaaaa tattctcatt ttattagtgg
     1501 aagtgagtgt atgccagcgg ccgcggcgcc aatgcattgg gcccggtacc cagcttttgt
     1561 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     1621 tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat aaagtgtaaa
     1681 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1741 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1801 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1861 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1921 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1981 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2041 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2101 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2161 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2221 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2281 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2341 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2401 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2461 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2521 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2581 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2641 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2701 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2761 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2821 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2881 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2941 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3001 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3061 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3121 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3181 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3241 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3301 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3361 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3421 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3481 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3541 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3601 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3661 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3721 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     3781 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     3841 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     3901 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     3961 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     4021 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     4081 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     4141 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     4201 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4261 cctcttcgct attacgccag tcgaccgcca attcaatatg gcgtatatgg actcatgcca
     4321 attcaatatg gtggatctgg acctgtgcca attcaatatg gcgtatatgg actcgtgcca
     4381 attcaatatg gtggatctgg accccagcca attcaatatg gcggacttgg caccatgcca
     4441 attcaatatg gcggacctgg cactgtgcca actggggagg ggtctacttg gcacggtgcc
     4501 aagtttgagg aggggtcttg gccctgtgcc aagtccgcca tattgaattg gcatggtgcc
     4561 aataatggcg gccatattgg ctatatgcca ggatcaatat ataggcaata tccaatatgg
     4621 ccctatgcca atatggctat tggccaggtt caatactatg tattggccct atgccatata
     4681 gtattccata tatgggtttt cctattgacg tagatagccc ctcccaatgg gcggtcccat
     4741 ataccatata tggggcttcc taataccgcc catagccact cccccattga cgtcaatggt
     4801 ctctatatat ggtctttcct attgacgtca tatgggcggt cctattgacg tatatggcgc
     4861 ctcccccatt gacgtcaatt acggtaaatg gcccgcctgg ctcaatgccc attgacgtca
     4921 ataggaccac ccaccattga cgtcaatggg atggctcatt gcccattcat atccgttctc
     4981 acgcccccta ttgacgtcaa tgacggtaaa tggcccactt ggcagtacat caatatctat
     5041 taatagtaac ttggcaagta cattactatt ggaagtacgc cagggtacat tggcagtact
     5101 cccattgacg tcaatggcgg taaatggccc gcgatggctg ccaagtacat ccccattgac
     5161 gtcaatgggg aggggcaatg acgcaaatgg gcgttccatt gacgtaaatg ggcggtaggc
     5221 gtgcctaatg ggaggtctat ataagcaatg ctcgtttagg gaac
//
