LOCUS       sequence-32474-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 cand142
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    103..127
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(542..566)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             592..633
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(673..692)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    717..765
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 atagcagact ctctggctac tagagaaccc actgccttac tggcttatcg aaattaatac
       61 gactcactat agggagaccc aagctggcta gttaagctat caacaagttt gtacaaaaaa
      121 gcaggctcat atggtggcag cattcctagc ttcaggttgg tctgggtgat gctgggccgg
      181 caggaagcct ggtctcgatg cttctcagtt ccataattca tgctggaaat tggccggcat
      241 gtcccgcccc agccccagcg gtgatgtatg ggcgccgtcg cagtaagaaa aaggcagagt
      301 aaatgtgtaa tttctccctt gacggccccc ggccgctggg cgatccttct tgttgctgcc
      361 gcgtggggac ggcccgtccg ccacactccg tcttcccgcc acccgccttg atgtctccat
      421 ttcattactg tgcgaccatt catcacaccc atggccaggg tgactttgcc ggcactttcg
      481 tgtgtgtggg agatgtgacc caggccataa tcactagtga caaggcacaa ggcttaagct
      541 tacccagctt tcttgtacaa agtggttgat ctagagggcc cgcggttcga aggtaagcct
      601 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg agtttaaacg
      661 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
      721 aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa cgcggggttc
      781 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
      841 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaag
//
