LOCUS       sequence-324746-        1293 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  LacI expression cassette (A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Greco FV, Pandi A, Erb TJ, Grierson CS, Gorochowski TE
  TITLE     Harnessing the central dogma for stringent multi-level control of 
            gene expression.
  JOURNAL   Nat Commun. 2021 Mar 19;12(1):1738. doi: 10.1038/s41467-021-21995-7.
  PUBMED    33741937
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-19"; volume: "12(1):1738. doi"; pages: " 
            10.1038/s41467-021-21995-7"
COMMENT     Sequence Label: pA-01
FEATURES             Location/Qualifiers
     source          1..1293
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(68..1150)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYMTVSRVVNQASHVSAKTREKVEAAMVEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIYSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1112..1131
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ggtctcagtg aagtgaaaag aaaaaaggcc gcagagcggc ctttttagtt agatcggacc
       61 ccacatctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      121 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
      181 ccagtgagac tggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
      241 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
      301 ggatataaca tgagctatct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
      361 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
      421 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
      481 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
      541 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
      601 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtcct
      661 catgggagta aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
      721 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
      781 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
      841 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
      901 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
      961 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     1021 ccaccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     1081 ccacgcggga aacggtcata taagagacac cggcatactc tgcgacatcg tataacgtta
     1141 ctggtttcat attcacctcc atgaattgac tctcttccgg gcgctatcat gccataccgc
     1201 gaaaggtttt tcaccattcg atggcgcgcc gcggctggga atctgagaca tgagtcaaga
     1261 tatttgctcg gtaacgtatg ctggagtgag acc
//
