LOCUS       sequence-324755-        8387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral plasmid for turq-3xFLAG-TREX1 D18N 
            (catalytically-deficient).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
  TITLE     ER-directed TREX1 limits cGAS activation at micronuclei.
  JOURNAL   Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037.
  PUBMED    33476576
REFERENCE   2  (bases 1 to 8387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037."
COMMENT     Sequence Label: pQCXIZ-turq-3xFLAG-TREX1 D18N
FEATURES             Location/Qualifiers
     source          1..8387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             205..380
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    443..642
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             843..1259
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1189..1211
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1227..1243
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1296..1599
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1600..1803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1800..1824
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(1942..1944,1945..1947,1948..2658)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFAHYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1987..2008)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2248..2267)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2595..2616
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2698..2763
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    3710..4276
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3870..3887)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4097..4116
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4293..4667
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             4785..5210
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(5313..5335)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5471..5491)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5476..5805
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5656..5791
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5827..5846
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5927..5944)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6098..6686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6178..6197)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6857..7648,7649..7717))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7480..7499
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7718..7822)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7890..7908
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        8008..8387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcaataa aagagcccac aacccctcac tcggcgcgcc
      241 agtcttccga tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt
      301 tgcatccgaa tcgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
      361 cacgacgggg gtctttcatt tgggggctcg tccgggattt ggagacccct gcccagggac
      421 caccgaccca ccaccgggag gtaagctggc cagcaactta tctgtgtctg tccgattgtc
      481 tagtgtctat gtttgatgtt atgcgcctgc gtctgtacta gttagctaac tagctctgta
      541 tctggcggac ccgtggtgga actgacgagt tctgaacacc cggccgcaac cctgggagac
      601 gtcccaggga ctttgggggc cgtttttgtg gcccgacctg aggaagggag tcgatgtgga
      661 atccgacccc gtcaggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc
      721 ctccgtctga atttttgctt tcggtttgga accgaagccg cgcgtcttgt ctgctgcagc
      781 gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt
      841 agggccagac tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc
      901 ggatcgctca caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg
      961 cagaatggcc aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca
     1021 tcacccaggt taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc
     1081 cctacatcgt gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg
     1141 tacaccctaa gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc
     1201 ctcgttcgac cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccg
     1261 gaattgaaga tctgggggat cgatcctcta gagtccgtta cataacttac ggtaaatggc
     1321 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     1381 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     1441 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     1501 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     1561 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     1621 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     1681 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     1741 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     1801 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     1861 agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacggacctg
     1921 caggaattga tccgcggccg catggtgagc aagggcgagg agctgttcac cggggtggtg
     1981 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     2041 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2101 ctgcccgtgc cctggcccac cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc
     2161 cactaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2221 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2281 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2341 gacggcaaca tcctggggca caagctggag tacaactact ttagcgacaa cgtctatatc
     2401 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     2461 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2521 gtgctgctgc ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac
     2581 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     2641 atggacgagc tgtacaaggg accggtaggc ctcgtacgct taattaacgg atccatggac
     2701 tacaaggacc acgacggtga ctacaaggac cacgacatcg actacaagga cgacgacgac
     2761 aagggctcgc aggccctgcc cccggggccc atgcagaccc tcatcttttt caacatggag
     2821 gccactggct tgcccttctc ccagcccaag gtcacggagc tgtgcctgct ggctgtccac
     2881 agatgtgccc tggagagccc ccccacctct caggggccac ctcccacagt tcctccacca
     2941 ccgcgtgtgg tagacaagct ctccctgtgt gtggctccgg ggaaggcctg cagccctgca
     3001 gccagcgaga tcacaggtct gagcacagct gtgctggcag cgcatgggcg tcaatgtttt
     3061 gatgacaacc tggccaacct gctcctagcc ttcctgcggc gccagccaca gccctggtgc
     3121 ctggtggcac acaatggtga ccgctacgac ttccccctgc tccaagcaga gctggctatg
     3181 ctgggcctca ccagtgctct ggatggtgcc ttctgtgtgg atagcatcac tgcgctgaag
     3241 gccctggagc gagcaagcag cccctcagaa cacggcccaa ggaagagcta cagcctaggc
     3301 agcatctaca ctcgcctgta tgggcagtcc cctccagact cgcacacggc tgagggtgat
     3361 gtcctggccc tgctcagcat ctgtcagtgg agaccacagg ccctgctgcg gtgggtggat
     3421 gctcacgcca ggcctttcgg caccatcagg cccatgtatg gggtcacagc ctctgctagg
     3481 accaagccaa gaccatctgc tgtcacaacc actgcacacc tggccacaac caggaacact
     3541 agtcccagcc ttggagagag caggggtacc aaggatcttc ctccagtgaa ggaccctgga
     3601 gccctatcca gggaggggct gctggcccca ctgggtctgc tggccatcct gaccttggca
     3661 gtagccacac tgtatggact atccctggcc acacctgggg agtaggaatt ccgccccccc
     3721 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     3781 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     3841 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     3901 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     3961 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     4021 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     4081 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     4141 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     4201 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     4261 aaaaacacga tgataagctt gatcacgcgt ccatggccaa gttgaccagt gccgttccgg
     4321 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     4381 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     4441 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     4501 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     4561 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     4621 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgagat atcatcagtg
     4681 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca
     4741 tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     4801 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     4861 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagggtc gaccctagag
     4921 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     4981 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     5041 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
     5101 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     5161 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
     5221 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc
     5281 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     5341 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     5401 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtag
     5461 atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5521 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5581 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5641 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5701 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     5761 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tactggctta
     5821 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     5881 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     5941 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6001 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6061 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6121 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6181 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6241 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6301 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6361 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6421 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6481 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6541 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6601 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6661 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6721 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6781 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6841 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6901 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6961 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7021 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7081 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7141 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     7201 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7261 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7321 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7381 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7441 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     7501 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7561 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7621 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7681 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7741 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7801 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     7861 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     7921 aagaattagc ttggccattg catacgttgt atccatatca taatatgtac atttatattg
     7981 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
     8041 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     8101 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     8161 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     8221 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     8281 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     8341 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatg
//
