LOCUS       sequence-324756-        9301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral plasmid for GFP-TREX1 R128A R174A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
  TITLE     ER-directed TREX1 limits cGAS activation at micronuclei.
  JOURNAL   Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037.
  PUBMED    33476576
REFERENCE   2  (bases 1 to 9301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037."
COMMENT     Sequence Label: pLENTI CMV GFP TREX1 R128A R174A BLAST
FEATURES             Location/Qualifiers
     source          1..9301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1714..2017
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2018..2221
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2172..2192
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2218..2242
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2311..2320
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2317..2319,2320..2322,2323..3033)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2317..2319,2320..2322,2323..3033)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2362..2383)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2623..2642)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2970..2991
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3043..3067
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4021..4609
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4074..4094)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4492..4503)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4660..4680)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4665..4994
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4845..4980
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5042..5089
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5108..5506
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5593..5826
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5898..6019
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5935..5954)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5989..6008
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6059..6194
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6214..6233)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6215..6233)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6243..6260)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6243..6259)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6252..6274)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6401..6856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6488..6507)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6698..6719
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6882..6986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6987..7055,7056..7847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7205..7224)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8018..8606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8507..8526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8760..8777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8894..8915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8930..8935,8936..8953,8954..8960)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8968..8984
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8973..8995
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8992..9008
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8992..9008
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9027..9047
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9029..9047
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9075..9301
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc
     1741 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1801 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1861 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1921 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1981 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     2041 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     2101 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     2161 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2221 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     2281 aagacaccga ctctagagga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
     2341 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     2401 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     2461 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     2521 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     2581 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     2641 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     2701 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     2761 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     2821 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     2881 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     2941 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     3001 gggatcactc tcggcatgga cgagctgtac aagaccggat caacaagttt gtacaaaaaa
     3061 gcaggctggg gctcgcaggc cctgcccccg gggcccatgc agaccctcat ctttttcgac
     3121 atggaggcca ctggcttgcc cttctcccag cccaaggtca cggagctgtg cctgctggct
     3181 gtccacagat gtgccctgga gagccccccc acctctcagg ggccacctcc cacagttcct
     3241 ccaccaccgc gtgtggtaga caagctctcc ctgtgtgtgg ctccggggaa ggcctgcagc
     3301 cctgcagcca gcgagatcac aggtctgagc acagctgtgc tggcagcgca tgggcgtcaa
     3361 tgttttgatg acaacctggc caacctgctc ctagccttcc tgcggcgcca gccacagccc
     3421 tggtgcctgg tggcacacaa tggtgacgcc tacgacttcc ccctgctcca agcagagctg
     3481 gctatgctgg gcctcaccag tgctctggat ggtgccttct gtgtggatag catcactgcg
     3541 ctgaaggccc tggagcgagc aagcagcccc tcagaacacg gcccagcgaa gagctatagc
     3601 ctaggcagca tctacactcg cctgtatggg cagtcccctc cagactcgca cacggctgag
     3661 ggtgatgtcc tggccctgct cagcatctgt cagtggagac cacaggccct gctgcggtgg
     3721 gtggatgctc acgccaggcc tttcggcacc atcaggccca tgtatggggt cacagcctct
     3781 gctaggacca agccaagacc atctgctgtc acaaccactg cacacctggc cacaaccagg
     3841 aacactagtc ccagccttgg agagagcagg ggtaccaagg atcttcctcc agtgaaggac
     3901 cctggagccc tatccaggga ggggctgctg gccccactgg gtctgctggc catcctgacc
     3961 ttggcagtag ccacactgta tggactatcc ctggccacac ctggggagta gttagtaatc
     4021 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4081 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4141 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4201 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4261 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4321 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4381 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     4441 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4501 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4561 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattctg
     4621 cagatatccg gttagtaatg agtttggaat taattctgtg gaatgtgtgt cagttagggt
     4681 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     4741 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4801 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     4861 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     4921 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     4981 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     5041 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     5101 ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     5161 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     5221 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     5281 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     5341 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     5401 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     5461 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac aattcgagct
     5521 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5581 aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg ctttttgctt
     5641 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5701 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5761 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5821 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5881 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5941 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6001 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     6061 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6121 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6181 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     6241 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     6301 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     6361 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     6421 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6481 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6541 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6601 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6661 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6721 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6781 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6841 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6901 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6961 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7021 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7081 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7141 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7201 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7261 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7321 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7381 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7441 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7501 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7561 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7621 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7681 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7741 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7801 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7861 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7921 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7981 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8041 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8101 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8161 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8221 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8281 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8341 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8401 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8461 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8521 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8581 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8641 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8701 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8761 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     8821 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8881 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     8941 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9001 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     9061 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     9121 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     9181 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     9241 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     9301 c
//
