LOCUS       sequence-32476-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 cand316
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(513..537)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             563..604
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(644..663)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    688..736
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ctcntggcta ctagagaacc cactgcttac tggcttatcg aaattaatac gactcaccta
       61 tagggagacc caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctca
      121 tatgcccctg aagttggctc actatcgtcg ctggagaccc cagcagaggg acgctgccca
      181 ggctgcgcag cccgcgagcc cgagcgtcac gcgcacttgc gcgcaccgcc cgcctctggc
      241 caggacgctt atacatccgg agggtgggca gtgcccgctc ctccgacctc cctgcgcacc
      301 ggccgggcgc agggagggcc gggggcggag cctggccagc cgagggagcc gaggacacga
      361 ggacatgttg gagaacttca ggccactggg tcgtatgctg ccccccggca cccagcagcc
      421 agcctgcctg ccgggagaag ctctatggcc cgaccaggct gggcagagtg aggccgaggc
      481 ccgccgctct gcctggtttc taggccaagc ttacccagct ttcttgtaca aagtggttga
      541 tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
      601 tacgcgtacc ggttagtaat gagtttaaac gggggaggct aactgaaaca cggaaggaga
      661 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt
      721 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
      781 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
      841 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
      901 aca
//
