LOCUS       sequence-324760-       10352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtPIP6:T35s
FEATURES             Location/Qualifiers
     source          1..10352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             118..1191
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1257..1451
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        1734..1838
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1839..2633
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2804..3392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3293..3312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3515..3539
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      5792..6044
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    7495..7669
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        7769..7948
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7970..8764
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8024..8043)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8634..8653
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      8777..9029
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9124..9148
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9412..10041
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
       61 ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
      121 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
      181 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
      241 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
      301 gccggtgcgt cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
      361 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
      421 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
      481 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
      541 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
      601 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
      661 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
      721 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
      781 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
      841 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
      901 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
      961 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
     1021 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
     1081 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
     1141 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
     1201 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaagctt ctttcctgtg
     1261 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
     1321 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
     1381 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
     1441 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
     1501 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
     1561 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
     1621 ccactcgacc gccggcgccc acatcaaggc accgagctcc catcccccca ggggctgcgc
     1681 ccctcggccg cgaacggcct caccccaaaa atggcagcgc tggccaattc gtgcgcggaa
     1741 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1801 cttgataaat gcttcaataa tattgaaaaa ggaagagtat ggctaaaatg agaatatcac
     1861 cggaattgaa aaaactgatc gaaaaatacc gctgcgcaaa agatacggaa ggaatgtctc
     1921 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
     1981 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
     2041 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
     2101 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
     2161 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
     2221 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
     2281 ataacgatct ggccgatgtg gattgcgaaa actgggaaga ggacactcca tttaaagatc
     2341 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
     2401 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
     2461 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga
     2521 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
     2581 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagctgtcag
     2641 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2701 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2761 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2821 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2881 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2941 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3001 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3061 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3121 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3181 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3241 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3301 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3361 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3421 tcctgtggtt ggcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata
     3481 tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa
     3541 acactgatag tttaaactcc ctcggtcaca tgtgcatcct cgtaaggagg acgtcgttgt
     3601 ggttggtgct ttccttacat tctgagcctc tttccttcta atccactcat ctgcatcttc
     3661 ttgtgtcctt actaatacct cattggttcc aaattccctc cctttaagca ccagctcgtt
     3721 tctgttcttc cacagcctcc caagtatcca agggactaaa gcctccacat tcttcagatc
     3781 aggatattct tgtttaagat gttgaactct atggaggttt gtatgaactg atgatctagg
     3841 accggataag ttcccttctt catagcgaac ttattcaaag aatgttttgt gtatcattct
     3901 tgttacattg ttattaatga aaaaatatta ttggtcattg gactgaacac gagtgttaaa
     3961 tatggaccag gccccaaata agatccattg atatatgaat taaataacaa gaataaatcg
     4021 agtcaccaaa ccacttgcct tttttaacga gacttgttca ccaacttgat acaaaagtca
     4081 ttatcctatg caaatcaata atcatacaaa aatatccaat aacactaaaa aaattaaaag
     4141 aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact
     4201 gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat
     4261 aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc
     4321 aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa
     4381 aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttaagga
     4441 aattgtggtt gtcggacgaa gtccagtaat aaacggcgtc aaagtggttg cagccggcac
     4501 acacgagtcg tgtttatcaa ctcaaagcac aaatactttt cctcaaccta aaaataaggc
     4561 aattagccaa aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca ttttattatt
     4621 agctattgct tcaccgcctt agctttctcg tgacctagtc gtcctcgtct tttcttcttc
     4681 ttcttctata aaacaatacc caaagagctc ttcttcttca caattcagat ttcaatttct
     4741 caaaatctta aaaactttct ctcaattctc tctaccgtga tcaaggtaaa tttctgtgtt
     4801 ccttattctc tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg tatatgttct
     4861 ttggtttaga ttctgttaat cttagatcgt agacgatttt ctgggtttga tcgttagata
     4921 tcatcttaat tctcgattag ggtttcatag atatcatccg atttgttcaa ataatttgag
     4981 ttttgtcgaa taattactct tcgatttgtg atttctatct agatctggtg ttagtttcta
     5041 gtttgtgcga tcgaatttgt cgattaatct gagtttttct gattaacaga tgcagatctt
     5101 aatggggtca tccaagaatg ttatcaagga gttcatgagg tttaaggttc gcatggaagg
     5161 aacggtcaat gggcacgagt ttgaaataga aggcgaagga gaggggaggc catacgaagg
     5221 ccacaatacc gtaaagctta aggtaaccaa ggggggacct ttgccatttg cttgggatat
     5281 tttgtcacca caatttcagt atggaagcaa ggtatatgtc aagcaccctg ccgacatacc
     5341 agactataaa aagctgtcat ttcctgaagg atttaaatgg gaaagggtca tgaactttga
     5401 agatggtggc gtcgttactg taacccagga ttccagtttg caggatggct gtttcatcta
     5461 caaggtcaag ttcattggcg tgaactttcc ttccgatgga cctgttatgc aaaagaaaac
     5521 aatgggctgg gaagccagca ctgagcgttt gtatcctcgt gatggcgtgt tgaaaggaga
     5581 gattcataag gctctgaagc tgaaagacgg tggtcattac ctagttgaat tcaaaagtat
     5641 ttacatggca aagaagcctg tgcagctacc agggtactac tatgttgact ccaaactgga
     5701 tataacaagc cacaacgaag attatacaat cgttgagcag tatgaaagaa ccgagggacg
     5761 ccaccatctg ttcctttaag cttgtcaagc agatcgttca aacatttggc aataaagttt
     5821 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     5881 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     5941 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     6001 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgacgct gagctcgaat
     6061 tctagtggag ggagagagga ttttgaggaa ataattaatt gaattacttg tattattgat
     6121 aaagtaattt agataagttg ttagtacaac ttattgcaac tatggataga caaaaatcac
     6181 ttattttaag gggagaagta tataacaact tattataaaa tttctgagca gccggcctcc
     6241 tccaatcaat catagagagt ggagcccatt ctggaaaacc aaggaacccc caacttgcac
     6301 tggtgcggtg gcccaattca aaaaaaacgg ggcccagcaa agtaacgccg ttgtaacgat
     6361 ttatcaatcc aaactcaaaa ggcggcgaga tgcgtttaac tccgtgaaat taacaaaccg
     6421 ccaacaactt gcaatttgca actaccgttt ccaagaagaa ctcaaccaca caacgtatcc
     6481 tatcccaaac cacacgcaac tagtgacgcg tcataaggac acgtgtcaca atttgactgg
     6541 ttaataattt caccgctttt gctataaatt acctccaatc cccttagctt cttcacaatt
     6601 cagttcccaa ccctaacaat tcttgttcat atcgcttctc tctactttca aggtatgatc
     6661 caatttctct cttctttctc tgtaatcctt tcgttgagtt ttgtttccga tcaatcatag
     6721 gtagttttct tgtttcgaag catgagatct aggaattttt tgtgattttc caaaattgag
     6781 atcggtttga aattgaattt tacagcttga atctcagatc ttgttttatc aatgttttcg
     6841 atggcttgcg atgtagatct atgataattg tggttcagtt ttgttaggaa tcgatttcgg
     6901 tttagcaatt gcagattaat tagggtttcc aattgaattc ttcagatccg ttatggaatt
     6961 atgtcaaata atttattcaa attggaaatt attgttagat ccactcttaa tctgtttgat
     7021 ccaagcttca attagggttt tcacttgttt caatttcttg ttatggattc tgatttatct
     7081 gttgatgtta gatccactct taatctgttt gatccaagcg ttaattaggg ttttcacttg
     7141 tttcaatttc gtgtgttgga ttctgattta tctgttgttg atgtgattac agaatggttg
     7201 gaaaatcagt caaattatat tactttgtta ttattgttat tattttgttt gagttgagct
     7261 tccaattcca acatgggatg gctcggccac taaaacagca taatgatgaa gatgatcagc
     7321 ataatattaa tactaatagt gttgtaataa taagcagtag ttcagcacat aataagaaag
     7381 tacttgagga gattaacaac tctggtccta gccctggaga aggccataag caacaactac
     7441 cagatggcta tcatcattaa gcttctctag ctagagtcga tcgacaagct cgagtttctc
     7501 cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc
     7561 atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca
     7621 ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcg ctaagcttga
     7681 attcttgcgg agagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
     7741 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc
     7801 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
     7861 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt
     7921 tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaaa tgattgaaca
     7981 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     8041 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     8101 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aagacgaggc
     8161 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     8221 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     8281 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     8341 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagg
     8401 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     8461 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgcccgacg gcgaggatct
     8521 cgtcgtgacc cagggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     8581 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     8641 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     8701 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     8761 ctgagcttgt caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     8821 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     8881 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     8941 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     9001 gcgcgcggtg tcatctatgt tactagatcg acgctggatc cgaattcggc agggcccact
     9061 gcatccaccc cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
     9121 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
     9181 cggcacaaaa tcaccactcg atacaggcag cccatcagcc gtgcggctgc atgaaatcct
     9241 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
     9301 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     9361 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     9421 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     9481 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     9541 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     9601 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     9661 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     9721 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     9781 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     9841 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     9901 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     9961 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
    10021 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
    10081 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
    10141 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
    10201 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
    10261 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
    10321 ggaggcggca tggaaaatca agaacaacca gg
//
