LOCUS       sequence-324763-        2415 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression of NarL in E. coli for cell-free nitrate biosensing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2415)
  TITLE     Sensitizing cell-free molecular detection using cascaded genetic 
            circuitry
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJBL7041
FEATURES             Location/Qualifiers
     source          1..2415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     633..652
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        633..651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1416..1434)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1430..1477
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1495..2310)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2218..2237
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2302..2321
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg atcccgcgaa attaatacga ctcactatag ggagaccaca
      661 acggtttccc taaggatttt ttctagaaga attatataca acaaatgagt aatcaggaac
      721 cggctactat cctgctgatt gacgatcacc cgatgctgcg aactggcgta aaacagctta
      781 tcagtatggc accagatatc accgtggttg gcgaagcgag taatggcgaa cagggtattg
      841 aactggcgga gtctcttgat cccgatctga tcctgttaga tctcaatatg cccggcatga
      901 acggtctgga aacgctggat aaactgcgcg aaaagtccct ctcagggcgc attgtggtat
      961 tcagcgtctc taaccatgaa gaagatgtgg tcaccgcact gaaacgcggc gcggatggct
     1021 atctgttaaa agatatggaa ccggaagatc tgctgaaagc attgcatcag gcagctgctg
     1081 gcgaaatggt attaagccct gatatcctta aacgtctgca agaaatccaa tttgagcgga
     1141 tgaaaaagca gcgcaatgag acgcagctga cagaaaagga agtcattgtt ctaaaagcaa
     1201 ttgctaaagg tcttaaaagc aaagcgattg cctttgattt gggcgtctct gagcgaacag
     1261 taaagtccag attaacgtcc atttacaata aattaggcgc gaattcaaga actgaggcag
     1321 taacgattgc catgcaaaaa ggtattctga caatagacaa ctaagtcgac cggctgctaa
     1381 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
     1441 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaagccaatt ctgattagaa
     1501 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     1561 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     1621 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     1681 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     1741 cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt
     1801 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     1861 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     1921 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     1981 taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg
     2041 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     2101 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     2161 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     2221 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcttcga
     2281 gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc
     2341 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
     2401 ttgagacaca acgtg
//
