LOCUS       sequence-324764-        2216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression of MerR in E. coli for cell-free mercury sensing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2216)
  TITLE     Sensitizing cell-free molecular detection using cascaded genetic 
            circuitry
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJBL7042
FEATURES             Location/Qualifiers
     source          1..2216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     633..652
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        633..651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             683..705
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             713..724
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(1217..1235)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1231..1278
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1296..2111)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2019..2038
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2103..2122
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg atcccgcgaa attaatacga ctcactatag ggagaccaca
      661 acggtttccc tctagaaata attttgttta actttaagaa ggagatatac ataaagagga
      721 gaaatactag atggaaaata atttggaaaa cctgaccatt ggcgtttttg ccaaggcggc
      781 cggggtcaac gtggagacaa tccgcttcta tcagcgcaag ggcctgttgc gggaaccgga
      841 caagccttac ggcagcatcc gccgctatgg ggaggcggac gtggttcggg tgaaattcgt
      901 gaaatcggca cagcggctgg ggttcagtct ggacgagatt gccgagctgt tgcggctcga
      961 cgatggcacc cactgcgagg aggccagcag cctggccgaa cacaagctca aggacgtgcg
     1021 cgagaagatg gccgacttgg cgcgcatgga aaccgtgctg tctgaactcg tgtgcgcctg
     1081 ccatgcacga aaggggaatg tttcctgccc gttgatcgcg tcactacagg gcgaagcagg
     1141 cctggcaagg tcagctatgc cttaggtcga ccggctgcta acaaagcccg aaaggaagct
     1201 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     1261 gtcttgaggg gttttttgct gaaagccaat tctgattaga aaaactcatc gagcatcaaa
     1321 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     1381 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     1441 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     1501 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
     1561 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     1621 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     1681 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     1741 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     1801 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     1861 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     1921 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     1981 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     2041 tataaatcag catccatgtt ggaatttaat cgcggcttcg agcaagacgt ttcccgttga
     2101 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     2161 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtg
//
