LOCUS       sequence-32477-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-122
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..52
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        33..51
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    81..105
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(409..433)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             459..500
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(540..559)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    584..632
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
       61 gctggctagt taagctatca acaagtttgt acaaaaaagc aggctcccta tgctccagtt
      121 tcagcgtttg gaaccactga ggagtctgtg acagacaacg gaggaatgtg aagggaaggt
      181 taataccctt gtgcctgaca gactttcctt agcagagctg tggagtgtga caatggtgtt
      241 tgtgtccaaa ccatcaaacg ccattatcac actaaatagc tactgctagg caatccgtcc
      301 actccacgcg tgacttgacg tctgttctct ccgagcaaga agttctgtct tgtgctagag
      361 ttctccattg tgtgtaatgt atgaataaaa tctggtcctc ttgtgcttac ccagctttct
      421 tgtacaaagt ggttgatcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
      481 tcctcggtct cgattctacg cgtaccggtt agtaatgagt ttaaacgggg gaggctaact
      541 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
      601 aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
      661 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
      721 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
      781 ggcaggccct gccatagcag atctgcgcag ctggggctct agggggtatc cccacgcgcc
      841 ctgta
//
