LOCUS       sequence-32478-e         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-133a-1
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(431..455)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             481..522
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(562..581)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    606..654
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct ggctagttaa gctatcaaca agtttgtaca aaaaagcagg ctgcctaaac
      121 acgtgaattt tctgtttaac aactggtaca ctagtgtggg aacctctaat acctgtcatg
      181 ctatatttct acaaaagagc atttaacctg tttggatcct acaccagcag cggcaatgct
      241 ttgctaaagc tggtaaaatg gaaccaaatc gcctcttcaa tggatttggt ccccttcaac
      301 cagctgtagc tatgcattga ttacacatgg acagccagtg ttttatgagc atgaattaca
      361 tctcaactta ctcaattcat acaaactgta actaaaagca aatacctagc aactgtcaac
      421 cttatttgga acccagcttt cttgtacaaa gtggttgatc tagagggccc gcggttcgaa
      481 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga
      541 gtttaaacgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
      601 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
      661 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
      721 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
      781 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc agatctgcgc agctggggct
      841 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggg tgtggtggtt
      901 acccgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgct
//
