LOCUS       sequence-32480-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-138-1
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(450..474)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             500..541
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(581..600)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    625..673
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tgagaaccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 ctggctagtt aagctatcaa caagtttgta caaaaaagca ggctcagagc cacctttgga
      121 tcatcaatca tctgccttcc caggaccacc cagcagtgat gcacgtagag cagaggtgca
      181 gtgatgcacg tacagcagag gcgacccctt ctccatactt cagagacctc tagcatggtg
      241 ttgtgggaca gctggtgttg tgaatcaggc cgttgccaat cagagaacgg ctacttcaca
      301 acaccagggc cacactgcac tgcaagcagc gagcagcaag caacaagcat ccagacacct
      361 gagcaccagg atgccagcaa gagcacctgc ctgtgaggaa acctcccagc ccaccccata
      421 ccccacccca ttatcccacc ctaccccaca cccagctttc ttgtacaaag tggttgatct
      481 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
      541 gcgtaccggt tagtaatgag tttaaacggg ggaggctaac tgaaacacgg aaggagacaa
      601 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt
      661 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
      721 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
      781 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagca
      841 gatctgcgca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
      901 gcggc
//
