LOCUS       sequence-32482-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-153
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    86..110
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(413..437)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             463..504
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(544..563)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    588..636
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tactagaaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
       61 cccaagctgg ctagttaagc tatcaacaag tttgtacaaa aaagcaggct ctctttccct
      121 ccctccctct tctcctctct gcacctcttg ctgtgttcca tgcatccact aacgctgctc
      181 actgtcaaac cgacaaaccc ttactttgcc agctacttag cggtggccgg tgtcattttt
      241 gtgacgttgc agctagtaat atgagcccag ttgcatagtc acaaaagtga tcattggaaa
      301 ctgtgactgc agcagggaca tgggggcccc tctctgagcc tctaacgacc tcacctgact
      361 ttgcttgctg ttcgtttgca tggcgactca ctcagtgacc aaagggttgg ttacccagct
      421 ttcttgtaca aagtggttga tctagagggc ccgcggttcg aaggtaagcc tatccctaac
      481 cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagtttaaac gggggaggct
      541 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
      601 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
      661 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
      721 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
      781 gggcggcagg ccctgccata gcagatctgc gcagctgggg ctctaggggg tatccccacg
      841 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      901 cactgg
//
