LOCUS       sequence-324828-       11780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  No promoter, C-terminal NLUC:3xFlag:10xHis tag, 
            attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP 
            backbone, SpcR for bacteria, HygR for plant).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11780)
  AUTHORS   Urquiza-Garcia U, Millar AJ
  TITLE     Expanding the bioluminescent reporter toolkit for plant science with
            NanoLUC.
  JOURNAL   Plant Methods. 2019 Jul 8;15:68. doi: 10.1186/s13007-019-0454-4. 
            eCollection 2019.
  PUBMED    31316580
REFERENCE   2  (bases 1 to 11780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2019-07-8"; volume: "15:68. doi"; pages: " 
            10.1186/s13007-019-0454-4. eCollection 2019"
COMMENT     Sequence Label: pGWB501NL3F10H
FEATURES             Location/Qualifiers
     source          1..11780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(499..504,505..522,523..529)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             583..1242
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(649..668)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1584..1889
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1768..1787
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1930..2054)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2065..2577
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             2587..2652
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2653..2682
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     terminator      2702..2954
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2987..3003)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2987..3003)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3000..3022)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3011..3027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3035..3041,3042..3059,3060..3065))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3080..3101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3137..3389)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3540..4565)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4617..4796)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5302..5326
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5847..6638
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6884..7472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7373..7392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7626..7643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7658..7798
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7724..7743)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7884..7906
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8142..8336
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8402..9475)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9904..10533)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcatggtc ttcacactcg aagatttcgt tggggactgg
     2101 cgacagacag ccggctacaa cctggaccaa gtccttgaac agggaggtgt gtccagtttg
     2161 tttcagaatc tcggggtgtc cgtaactccg atccaaagga ttgtcctgag cggtgaaaat
     2221 gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag gtctgagcgg cgaccaaatg
     2281 ggccagatcg aaaaaatttt taaggtggtg taccctgtgg atgatcatca ctttaaggtg
     2341 atcctgcact atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc
     2401 ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc
     2461 ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg
     2521 ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgcct
     2581 cgagttgatt acaaggatca tgatggtgat tacaaggatc acgacatcga ctacaaggat
     2641 gacgatgaca agcatcacca tcaccatcac catcaccatc actgagagct cgaatttccc
     2701 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     2761 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     2821 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     2881 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     2941 tatgttacta gatcgggaat tggttccgga accaattcgt aatcatgtca tagctgtttc
     3001 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3061 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aggctgaatt aggcgcgcct
     3121 atttctatta attcccgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     3181 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     3241 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     3301 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
     3361 aaactttatt gccaaatgtt tgaacgatcg gggaaattcg ggggatcgat cccgcgaact
     3421 gtggacgaga actgtgccac caagcgtaag gccgttctct cgcatttgcc ttgctaggct
     3481 cgcgcgagtt gctggctgag gcgttctcga aatcagctct tgttcggtcg gcatctactc
     3541 tattcctttg ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct
     3601 acacagccat cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc
     3661 ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa
     3721 ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg
     3781 agccgcggcg atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc
     3841 atacaagcca accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg
     3901 aacatcgcct cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg
     3961 ttggagccga aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc
     4021 atcagctcat cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc
     4081 cagtgataca catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga
     4141 ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg
     4201 atcgcatcca tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg
     4261 tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat
     4321 gccccaatgt caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca
     4381 taacgatctt tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct
     4441 cctacatcga agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag
     4501 acgctgtcga acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt
     4561 ttcatatctt attgcccccc gggatcagat ctggattgag agtgaatatg agactctaat
     4621 tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct
     4681 agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct
     4741 tagctcatta aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc
     4801 agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct
     4861 taattctccg ctcatgatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc
     4921 atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc
     4981 ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa
     5041 gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca
     5101 ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag
     5161 cagcccttgc gccctgagtg cttgcggcag cgtgaagctg atccgtcgag attaatagaa
     5221 ataaattcag cctaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt
     5281 aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca
     5341 gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg
     5401 ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct
     5461 attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta
     5521 gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc
     5581 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     5641 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     5701 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     5761 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     5821 gtcgccctaa aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc
     5881 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     5941 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     6001 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     6061 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     6121 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     6181 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     6241 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     6301 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     6361 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     6421 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     6481 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     6541 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     6601 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca
     6661 agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt
     6721 gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag
     6781 ccctacacaa attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt
     6841 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6901 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6961 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7021 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7081 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7141 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7201 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7261 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7321 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7381 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7441 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7501 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7561 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7621 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     7681 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     7741 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     7801 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     7861 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     7921 accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg
     7981 cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag
     8041 cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag
     8101 gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg
     8161 ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt
     8221 ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt
     8281 acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa
     8341 agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac
     8401 attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg
     8461 ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca
     8521 gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga
     8581 tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga
     8641 gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg
     8701 ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact
     8761 ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg
     8821 ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg
     8881 tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc
     8941 ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct
     9001 tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt
     9061 cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg
     9121 gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg
     9181 ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa
     9241 aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg
     9301 gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg
     9361 cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt
     9421 catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga
     9481 ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac
     9541 gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg
     9601 ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt
     9661 tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc
     9721 cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga
     9781 cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg
     9841 cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt
     9901 aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc
     9961 gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac
    10021 gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac
    10081 ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct
    10141 tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat
    10201 gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac
    10261 agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc
    10321 cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc
    10381 ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc
    10441 ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc
    10501 gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt
    10561 actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat
    10621 caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc
    10681 agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg
    10741 ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa
    10801 aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct
    10861 cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc
    10921 tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat
    10981 gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc
    11041 gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg
    11101 ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg
    11161 caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg
    11221 gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta
    11281 ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc
    11341 gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct
    11401 cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct
    11461 ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg
    11521 cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa
    11581 gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca
    11641 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
    11701 ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa
    11761 atggacgaac ggataaacct
//
