LOCUS       sequence-32483-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-208a
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..52
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        33..51
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    81..105
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(438..462)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             488..529
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(569..588)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    613..661
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
       61 gctggctagt taagctatca acaagtttgt acaaaaaagc aggctgatgc aggaaagagc
      121 tttggggtgg gagaacagag gatggggaga cctggtgagc catgggagtg tttggtagaa
      181 agcctgagtc ctgcaacctg taacgcctgg ctggcccttg cttacttcct ttgacgggtg
      241 agcttttggc ccgggttata cctgacactc acgtataaga cgagcaaaaa gcttgttggt
      301 cagaggagct atcatcatgc agcctgggcc tgagggaagc gggtgggggt gcacagtcac
      361 acagatgtga tgtatgtgtg ggagagtctg agcagcctgt gatggacgtc tctgttctag
      421 gctccagtag ccctcacacc cagctttctt gtacaaagtg gttgatctag agggcccgcg
      481 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta
      541 gtaatgagtt taaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
      601 cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt
      661 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
      721 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
      781 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcana tctgcgcagc
      841 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcggggtgt
      901 ggtggtt
//
