LOCUS       sequence-324837-        9920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses  DCLK1 G511N in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Liu Y, Ferguson FM, Li L, Kuljanin M, Mills CE, Subramanian K, 
            Harshbarger W, Gondi S, Wang J, Sorger PK, Mancias JD, Gray NS, 
            Westover KD
  TITLE     Chemical Biology Toolkit for DCLK1 Reveals Connection to RNA 
            Processing.
  JOURNAL   Cell Chem Biol. 2020 Oct 15;27(10):1229-1240.e4. doi: 
            10.1016/j.chembiol.2020.07.011. Epub 2020 Aug 4.
  PUBMED    32755567
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2020.07.011"; journalName: "Cell Chem Biol"; 
            date: "2020-10-15- 15"; volume: "27"; issue: "10"; pages: 
            "1229-1240.e4"
COMMENT     Sequence Label: plenti_DCLK1 G511N
FEATURES             Location/Qualifiers
     source          1..9920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    625..649
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2838..2862)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2915..2956
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2980..3568
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3033..3053)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3451..3462)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3583..3899
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3750..3885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3947..3994
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4013..4411
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4498..4731
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4803..4924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4840..4859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4894..4913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4964..5099
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5119..5138)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5120..5138)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5148..5165)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5148..5164)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5157..5179)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5306..5761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5393..5412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5603..5624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5787..5891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5892..5960,5961..6752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6110..6129)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6923..7511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7412..7431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7665..7682
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7799..7820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7835..7840,7841..7858,7859..7865)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7873..7889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7878..7900
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7897..7913
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7897..7913
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7932..7952
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7934..7952
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7980..8206
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8207..8387
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8434..8559
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9052..9285
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9470..9514
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9781..9898
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tagaggatcc actagtccag
      601 tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggcta tgtccttcgg
      661 cagagacatg gagctggagc acttcgacga gcgggataag gcgcagagat acagccgagg
      721 gtcgcgggtg aacggcctgc cgagcccgac gcacagcgcc cactgcagct tctaccgcac
      781 ccgcacgctg cagacgctca gctccgagaa gaaggccaag aaagttcgtt tctatcgaaa
      841 cggagatcga tacttcaaag ggattgtgta tgccatctcc ccagaccggt tccgatcttt
      901 tgaggccctg ctggctgatt tgacccgaac tctgtcggat aacgtgaatt tgccccaggg
      961 agtgagaaca atctacacca ttgatgggct caagaagatt tccagcctgg accaactggt
     1021 ggaaggagag agttatgtat gtggctccat agagcccttc aagaaactgg agtacaccaa
     1081 gaatgtgaac cccaactggt cggtgaacgt caagaccacc tcggcttctc gggcagtgtc
     1141 ttcactggcc actgccaaag gaagcccttc agaggtgcga gagaataagg atttcattcg
     1201 gcccaagctg gtcaccatca tcagaagtgg cgtgaagcca cggaaagctg tcaggattct
     1261 gctgaacaag aaaacggctc attcctttga gcaggtcctc accgatatca ccgatgccat
     1321 caagctggac tcgggagtgg tgaaacgcct gtacacgttg gatgggaaac aggtgatgtg
     1381 ccttcaggac ttttttggtg atgatgacat ttttattgca tgtggaccgg agaagttccg
     1441 ttaccaggat gatttcttgc tagatgaaag tgaatgtcga gtggtaaagt ccacttctta
     1501 caccaaaata gcttcatcat cccgcaggag caccaccaag agcccaggac cgtccaggcg
     1561 tagcaagtcc cctgcctcca ccagctcagt taatggaacc cctggtagtc agctctctac
     1621 tccgcgctca ggcaagtcgc caagcccatc acccaccagc ccaggaagcc tgcggaagca
     1681 gaggagctct cagcatggcg gctcctctac gtcacttgcg tccaccaaag tctgcagctc
     1741 gatggatgag aacgatggcc ctggagaaga agtgtcggag gaaggcttcc agattccagc
     1801 tacaataacg gaacgatata aagtcggaag aacaatagga gatggaaatt ttgctgttgt
     1861 caaggaatgt gtagaaagat cgactgctag agagtacgct ctgaaaatta tcaagaaaag
     1921 caaatgtcga ggcaaagagc acatgatcca gaatgaagtg tctattttaa gaagagtgaa
     1981 gcatcccaat atcgttcttc tgattgagga gatggatgtg ccaactgaac tgtatcttgt
     2041 catggaatta gtaaaggggg gagacctttt tgatgccatt acttccacta acaaatacac
     2101 cgagagagac gccagtggga tgctgtacaa cctagccagc gccatcaaat acctgcatag
     2161 cctgaacatc gtccaccgta atatcaagcc agagaacctg ctggtgtatg agcaccaaga
     2221 tggcagcaaa tcactgaagc tgggtgactt tggactggcc accattgtag acggccccct
     2281 gtacacagtc tgtggcaccc caacatacgt ggctccagaa atcattgcag agactggata
     2341 cggcctcaag gtggacatct gggcagcagg tgtaatcact tatatcctgc tgtgtggttt
     2401 ccctccattc cgtggaagtg gtgatgacca ggaggtgctt tttgatcaga ttttgatggg
     2461 gcaggtggac tttccttctc catactggga taatgtttcc gattctgcaa aggagctcat
     2521 taccatgatg ctgttggtcg atgtagatca gcgattttct gctgttcaag tacttgagca
     2581 tccctgggtt aatgatgatg gcctcccaga aaatgaacat cagctgtcag tagctggaaa
     2641 gataaagaag catttcaaca caggccccaa gccgaatagc acagcagctg gagtttctgt
     2701 catagcactg gaccacgggt ttaccatcaa gagatcaggg tctttggact actaccagca
     2761 accaggaatg tattggataa gaccaccgct cttgataagg agaggcaggt tttccgacga
     2821 agacgcaacc aggatggacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg
     2881 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     2941 cggtctcgat tctacgcgta ccggttagta atgatcgaca atcaacctct ggattacaaa
     3001 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3061 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3121 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3181 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3241 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3301 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3361 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     3421 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3481 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3541 cggatctccc tttgggccgc ctccccgcct ggcgatggta ccggtgtgga aagtccccag
     3601 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3661 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3721 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3781 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     3841 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     3901 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     3961 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     4021 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     4081 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     4141 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     4201 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     4261 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     4321 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     4381 gctgccctct ggttatgtgt gggagggcta agcacaattc gagctcggta cctttaagac
     4441 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4501 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4561 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4621 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4681 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     4741 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     4801 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4861 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4921 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     4981 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5041 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5101 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     5161 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5221 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5281 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5341 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5401 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5461 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5521 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5581 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5641 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5701 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     5761 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5821 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5881 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5941 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6001 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6061 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6121 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6181 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6241 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6301 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6361 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6421 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6481 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6541 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6601 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6661 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6721 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6781 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6841 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6901 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6961 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7021 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7081 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7141 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7201 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7261 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7321 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7381 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7441 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7501 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7561 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7621 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7681 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7741 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7801 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7861 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7921 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     7981 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     8041 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     8101 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     8161 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     8221 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8281 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8341 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     8401 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     8461 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     8521 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     8581 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     8641 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     8701 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8761 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8821 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8881 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8941 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     9001 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     9061 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     9121 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     9181 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     9241 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     9301 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     9361 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9421 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9481 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     9541 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9601 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     9661 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     9721 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac
     9781 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     9841 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttat
     9901 cgataagctt gggagttccg
//
