LOCUS       sequence-32484-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-216a
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(426..450)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             476..517
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(557..576)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    601..649
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tggctactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
       61 agacccaagc tggctagtta agctatcaac aagtttgtac aaaaaagcag gcttgtgatt
      121 ttagcaccag gtcattacct tgtacattca ggaaacagac acagttctac ctagagcctc
      181 caagcacctc tgatcacaag aattttcttg caggtccaac ctggatggct atgagttggt
      241 ttaatctcag ctggcaactg tgagatgtcc ctatcattcc tcacagtggt ctctgggatt
      301 atgctaaaca gagcaatttc cttgaccttg tggacccaaa gatgatcatg acaacctgaa
      361 tcagaacacc aaaattgaat ttcatgcaga cctatgagag ttactgtctg tcctcaacct
      421 cttctaccca gctttcttgt acaaagtggt tgatctagag ggcccgcggt tcgaaggtaa
      481 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagttta
      541 aacgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
      601 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
      661 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
      721 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
      781 gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg
      841 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      901 agcgtgaccg ctacacttgc cagcgcccta gcgcccgct
//
