LOCUS       sequence-32485-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-217
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    86..110
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(451..475)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             501..542
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(582..601)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    626..674
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ctactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
       61 cccaagctgg ctagttaagc tatcaacaag tttgtacaaa aaagcaggct ggggagtttg
      121 ggggattagt atgacattgg attgctttat taaatacctt cataaaagct ttcctaacta
      181 caaatgaaaa agtaaaagag aatgcgttgc tgtgttggga aatatgaatt gttaaacata
      241 gtcattacag tttttgatgt tgcagatact gcatcaggaa ctgactggat aagacttaat
      301 ccccatcagt tcctaatgca ttgccttcag catctaaaca accaccagca aacacctcaa
      361 gttagacttg ctgtgatgtg tttttggtca tctgacagga gatctagctc taagggaaaa
      421 agttttcttg aagagaagta ggcccaagat acccagcttt cttgtacaaa gtggttgatc
      481 tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
      541 cgcgtaccgg ttagtaatga gtttaaacgg gggaggctaa ctgaaacacg gaaggagaca
      601 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt
      661 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
      721 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
      781 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
      841 anatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
      901 cgcggcgggt gtggtggtta ccccccag
//
