LOCUS       sequence-32486-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-223
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        37..55
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    85..109
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(467..491)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             517..558
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(598..617)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    642..690
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
       61 ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagcaggctt catgttagtg
      121 tctgccattt gtctcacact tagggtaaga tataatcacc ctattttttt ctctttccag
      181 ttgcacatct tccagcatgt tcttgctgcc cagtggaggt tcctgatctg gccatctgca
      241 gtgtcacgct ccgtgtattt gacaagctga gttggacact ctgtgtggta gagtgtcagt
      301 ttgtcaaata ccccaagtgt ggctcatgcc tatcagctcc aggtccagga cagagcacat
      361 agcctgctgc ctacatatga atgcttatga aacatgaagc tctctggtgt tattctatgt
      421 ctttaaggga actataacat ttattcagag aacatcagag taggaaaccc agctttcttg
      481 tacaaagtgg ttgatctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
      541 ctcggtctcg attctacgcg taccggttag taatgagttt aaacggggga ggctaactga
      601 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
      661 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
      721 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
      781 ccccacccca cccccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
      841 gcaggccct
//
