LOCUS       sequence-32487-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-224
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(440..464)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             490..531
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(571..590)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    615..663
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tggctactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
       61 agacccaagc tggctagtta agctatcaac aagtttgtac aaaaaagcag gcttggcttg
      121 gaattcttgc tcttctacta gacaactatg ggcctgcctc ttagtacttc agtttgtcca
      181 gataggtgga gccccatcat cagaactggc agaagagaca aggcccgggg gcttttaagt
      241 cactagtggt tccgtttagt agatggtttg tgcattgttt caaaatggtg ccctagtgac
      301 tacaaagccc cagagccagc atcatcggtg aagcaatggc agtaggtaag caacatctct
      361 tcctgtggag aaagaagtag gtcttttcct ttgactagaa actacttatt ctgaattcat
      421 agtgtctcca ctgtcagtta cccagctttc ttgtacaaag tggttgatct agagggcccg
      481 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
      541 tagtaatgag tttaaacggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
      601 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg
      661 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
      721 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
      781 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagca gatctgcgca
      841 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      901 tggtgg
//
