LOCUS       sequence-324871-       10214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human PFAS-mCherry fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Doigneaux C, Pedley AM, Mistry IN, Papayova M, Benkovic SJ, 
            Tavassoli A
  TITLE     Hypoxia drives the assembly of the multienzyme purinosome complex.
  JOURNAL   J Biol Chem. 2020 Jul 10;295(28):9551-9566. doi: 
            10.1074/jbc.RA119.012175. Epub 2020 May 21.
  PUBMED    32439803
REFERENCE   2  (bases 1 to 10214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.012175"; journalName: "J Biol Chem"; date: 
            "2020-07-10- 10"; volume: "295"; issue: "28"; pages: "9551-9566"
COMMENT     Sequence Label: pFGAMS-mCherry
FEATURES             Location/Qualifiers
     source          1..10214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     543..563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     589..613
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          753..885
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     868..887
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        930..948
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5019..5028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5025..5735
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5170..5188)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5438..5457
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5781..5902
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5792..5811)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5846..5865
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6088..6543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6175..6194)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6385..6406
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6660..7017
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6660..6851
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6683..6703)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6868..7003
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7068..7862
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7122..7141)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7732..7751
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7926..7974
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8015..8034)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8134..8156
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8194..8212)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8280..8384
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8385..8453,8454..9245)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8603..8622)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9416..10004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9905..9924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctgcgatcgc ccgccccgtt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      601 gaaccgtcag atcactagaa gctttattgc ggtagtttat cacagttaaa ttgctaacgc
      661 agtcagtgct tctgacacaa cagtctcgaa cttaagctgc agtgactctc ttaaggtagc
      721 cttgcagaag ttggtcgtga ggcactgggc aggtaagtat caaggttaca agacaggttt
      781 aaggagacca atagaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg
      841 cacctattgg tcttactgac atccactttg cctttctctc cacaggtgtc cactcccagt
      901 tcaattacag ctcttaaggc tagagtactt aatacgactc actataggct agcctcgaga
      961 tgtccccagt ccttcacttc tatgttcgtc cctctggcca tgagggggca gcccctggac
     1021 acactcggag gaaactgcaa gggaaactgc cagagctgca gggcgtcgag actgaactgt
     1081 gctacaacgt gaactggaca gctgaggccc tccccagtgc tgaggagaca aagaagctga
     1141 tgtggctgtt tggttgcccc ttactgctgg atgatgttgc tcgggagtcc tggctccttc
     1201 ctggctccaa tgacctgctg ctggaggtcg ggcccaggct gaacttctcc accccaacat
     1261 ccaccaacat cgtgtcagtg tgccgcgcca ctgggctggg gcctgtggat cgtgtggaga
     1321 ccacccggcg ctaccggctc tcgtttgccc accccccgtc agctgaggtg gaagccattg
     1381 ctctggctac cctgcacgac cggatgacag agcagcactt cccccatccc atccagagtt
     1441 tctcccctga gagcatgccg gaacccctca atggccctat caatatactg ggtgagggcc
     1501 ggcttgcgct ggagaaggcc aaccaggagc ttggtctggc tttagactct tgggacctag
     1561 acttctacac caagcgcttc caggagctac agcggaaccc gagcactgtg gaggcctttg
     1621 acttggcgca gtccaatagc gagcacagcc gacactggtt cttcaagggc cagctccacg
     1681 tggatgggca gaagctggtg cactcactgt ttgagtccat catgagcacc caggaatcct
     1741 cgaaccccaa caacgtcctc aaattctgtg ataacagcag tgcaatccag ggaaaggaag
     1801 tccgattcct acggcctgag gaccccacac ggccaagccg cttccagcaa cagcaagggc
     1861 tgagacatgt tgtcttcaca gcagagactc acaactttcc cacaggagta tgccccttta
     1921 gtggtgcaac cactggcaca gggggccgga ttcgagatgt ccagtgcaca ggccgcgggg
     1981 cccacgtggt ggctggcact gccggctatt gctttggaaa tctgcatatt ccaggttaca
     2041 atctgccctg ggaggatcca agcttccagt atcctgggaa ttttgcccgg cccctggagg
     2101 ttgccattga agccagtaat ggagcttctg actatggcaa caagtttggg gaaccagtgc
     2161 tggctggctt cgcccgctcc ttgggcctcc agctcccaga cggccagcgg cgtgagtgga
     2221 tcaagcccat catgtttagt gggggcattg ggtccatgga agctgaccac ataagcaagg
     2281 aggccccaga gccaggcatg gaagttgtaa aggttggagg tcccgtctac aggattggag
     2341 ttggaggtgg agctgcttca tctgtgcagg tgcagggaga taacaccagt gacctggact
     2401 ttggggctgt gcagcgagga gacccggaga tggaacagaa gatgaaccgt gtgatcaggg
     2461 cttgtgtgga ggcccccaag ggaaacccca tctgcagcct tcatgatcag ggcgctggtg
     2521 gcaatggcaa tgtcctaaaa gagctgagtg acccagctgg agccatcatt tacaccagcc
     2581 gcttccagct tggggaccca accctgaatg ccctggaaat ctggggggct gagtaccagg
     2641 aatcaaatgc tcttctgctg aggtccccca accgggactt cctgactcat gtcagtgccc
     2701 gtgaacgttg cccggcttgc ttcgtgggca ccatcactgg agaccggaga atagtgctgg
     2761 tggacgatcg ggagtgtcct gtcagaagaa atggccaggg ggatgccccc ccgacacccc
     2821 tgccaacccc tgtggacctg gagctcgaat gggtgctggg caagatgcct cggaaggagt
     2881 tcttcctgca gaggaagccc cccatgctgc agcctctggc cttgccccca gggctgagcg
     2941 tgcaccaggc tctggagagg gttctgaggc tgcccgccgt ggccagcaag cgctacctca
     3001 ccaataaggt ggaccgctcc gtgggaggcc tggtggccca gcagcagtgc gtggggcccc
     3061 tgcaaactcc tctggcagat gtagcggttg tggcactgag ccatgaggag ctcatagggg
     3121 ctgccacagc cttgggagaa cagccagtca agagcctgct ggacccaaaa gtcgccgccc
     3181 ggctggccgt ggccgaagcc ctcaccaacc tggtgtttgc tctggtcact gacctccggg
     3241 atgtgaagtg tagcgggaac tggatgtggg cagccaagct cccaggggag ggcgcagctt
     3301 tggcggatgc ctgtgaggct atggtggcag tgatggcagc cctgggtgtg gcagtggatg
     3361 gtggcaagga ctccctcagc atggctgctc gggttggcac tgagaccgtg cgggctcctg
     3421 ggtcactggt catctcagcc tatgccgtct gcccagacat cacagccact gtgaccccag
     3481 acctcaagca tcctgaaggg agaggccatc tgctctatgt ggctctgagc cctgggcagc
     3541 accggctcgg gggcacagct ctggcccagt gcttctccca gcttggggaa caccctccag
     3601 acctggacct tcctgagaac ttggtgcggg ccttcagcat cactcagggg ctgctgaaag
     3661 accgcctcct ctgctcaggc cacgatgtca gtgacggagg cctcgtcaca tgcctgctgg
     3721 agatggcctt tgctggaaat tgcgggctac aggtggatgt gcctgtcccc agggttgatg
     3781 tcctgtctgt gctgttcgct gaggagccag gcctcgtgct ggaggtgcag gagccagacc
     3841 tggcccaggt gctgaagcgt taccgggatg ctggcctcca ttgcctggag ctgggccaca
     3901 caggcgaggc cgggccccac gccatggtcc gggtgtcagt gaacggggct gtggttctgg
     3961 aggagcctgt tggggagctg cgagccctct gggaggagac gagtttccag ctggaccggc
     4021 tacaggcaga gcctcgctgt gtggcagagg aggaacgggg cctgagggag cggatggggc
     4081 ccagctattg cctgcccccc acctttccca aagcctccgt gccccgtgag cctggtggtc
     4141 ccagcccccg agtcgccatc ttgcgagagg agggcagtaa tggagaccgg gagatggccg
     4201 atgccttcca cttagctggg tttgaggtat gggacgtgac catgcaggac ctctgctctg
     4261 gggcaattgg gctggacact ttccgtggcg tggccttcgt gggcggcttc agctatgcag
     4321 atgtcctggg ctctgccaaa gggtgggcag ctgctgtgac ctttcatccc agggctgggg
     4381 ctgagctgag gcgcttccgg aagcggccag ataccttcag cctgggcgtg tgtaatggct
     4441 gtcaactgct ggctctgctc ggctgggtgg gaggcgaccc caatgaggat gctgcagaga
     4501 tgggccctga ctcccagcca gcccggccag gccttctgct acgccacaac ctgtctgggc
     4561 gctacgagtc tcgctgggcc agcgtgcgtg tggggcctgg gccagccctg atgctgcgag
     4621 ggatggaggg cgccgtgctg cccgtgtgga gtgcgcacgg ggaaggttac gtagcatttt
     4681 cttctccgga actccaagct cagattgagg ccaggggctt ggctccactg cactgggctg
     4741 atgatgacgg gaaccccaca gagcagtacc ctctgaatcc caatgggtcc ccagggggcg
     4801 tggctggcat ctgctcctgt gatggccgcc acctggctgt catgcctcac cctgagcggg
     4861 ccgttaggcc ttggcagtgg gcatggcgac cccctccatt tgatactctg accacctccc
     4921 cctggctcca gctctttatc aatgcccgaa actggaccct ggaagggagc tgccgaattc
     4981 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg
     5041 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     5101 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     5161 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     5221 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     5281 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     5341 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     5401 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     5461 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     5521 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     5581 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     5641 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     5701 actccaccgg cggcatggac gagctgtaca agtagcggcc gcttcccttt agtgagggtt
     5761 aatgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     5821 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     5881 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     5941 gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatccg
     6001 ataaggatcg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     6061 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     6121 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6181 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6241 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6301 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6361 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     6421 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     6481 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     6541 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgcgg
     6601 atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt aggtaccttc
     6661 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     6721 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     6781 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6841 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6901 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6961 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     7021 cttgattctt ctgacacaac agtctcgaac ttaaggctag agccaccatg attgaacaag
     7081 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     7141 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     7201 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     7261 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     7321 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     7381 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     7441 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     7501 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     7561 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     7621 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     7681 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     7741 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     7801 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     7861 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgatg
     7921 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
     7981 tagcgataag gatccgcgta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     8041 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     8101 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     8161 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     8221 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     8281 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8341 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     8401 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     8461 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     8521 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     8581 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     8641 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     8701 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     8761 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     8821 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8881 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8941 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     9001 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     9061 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9121 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9181 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9241 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9301 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9361 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     9421 atcctttttt tctgtgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     9481 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     9541 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     9601 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     9661 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     9721 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     9781 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9841 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9901 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9961 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    10021 cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga cagatcttca
    10081 atattggcca ttagccatat tattcattgg ttatatagca taaatcaata ttggctattg
    10141 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
    10201 atgaccgcca tgtt
//
