LOCUS       sequence-324872-        9323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral plasmid for GFP-TREX1 G306A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
  TITLE     ER-directed TREX1 limits cGAS activation at micronuclei.
  JOURNAL   Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037.
  PUBMED    33476576
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037."
COMMENT     Sequence Label: pLENTI CMV GFP-TREX1 G306A BLAST
FEATURES             Location/Qualifiers
     source          1..9323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1941..2244
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2245..2448
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2399..2419
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2445..2469
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2538..2547
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2544..2546,2547..2549,2550..3260)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2544..2546,2547..2549,2550..3260)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2589..2610)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2850..2869)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3197..3218
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3270..3294
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4270..4858
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4323..4343)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4741..4752)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4909..4929)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4914..5243
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5094..5229
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5291..5338
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5357..5755
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5842..6075
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6147..6268
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6184..6203)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6238..6257
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6308..6443
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6463..6482)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6464..6482)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6492..6509)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6492..6508)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6501..6523)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6650..7105
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6737..6756)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6947..6968
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7131..7235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7236..7304,7305..8096)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7454..7473)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8267..8855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8756..8775
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9009..9026
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9143..9164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9179..9184,9185..9202,9203..9209)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9217..9233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9222..9244
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9241..9257
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9241..9257
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9276..9296
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9278..9296
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt rgygscygaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1981 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2041 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2101 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2161 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2221 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2281 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2341 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2401 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2461 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
     2521 tagaggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     2581 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2641 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2701 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     2761 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2821 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2881 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2941 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3001 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3061 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3121 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3181 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3241 gcatggacga gctgtacaag accggatcaa caagtttgta caaaaaagca ggctggggct
     3301 cgcaggccct gcccccgggg cccatgcaga ccctcatctt tttcgacatg gaggccactg
     3361 gcttgccctt ctcccagccc aaggtcacgg agctgtgcct gctggctgtc cacagatgtg
     3421 ccctggagag cccccccacc tctcaggggc cacctcccac agttcctcca ccaccgcgtg
     3481 tggtagacaa gctctccctg tgtgtggctc cggggaaggc ctgcagccct gcagccagcg
     3541 agatcacagg tctgagcaca gctgtgctgg cagcgcatgg gcgtcaatgt tttgatgaca
     3601 acctggccaa cctgctccta gccttcctgc ggcgccagcc acagccctgg tgcctggtgg
     3661 cacacaatgg tgaccgctac gacttccccc tgctccaagc agagctggct atgctgggcc
     3721 tcaccagtgc tctggatggt gccttctgtg tggatagcat cactgcgctg aaggccctgg
     3781 agcgagcaag cagcccctca gaacacggcc caaggaagag ctatagccta ggcagcatct
     3841 acactcgcct gtatgggcag tcccctccag actcgcacac ggctgagggt gatgtcctgg
     3901 ccctgctcag catctgtcag tggagaccac aggccctgct gcggtgggtg gatgctcacg
     3961 ccaggccttt cggcaccatc aggcccatgt atggggtcac agcctctgct aggaccaagc
     4021 caagaccatc tgctgtcaca accactgcac acctggccac aaccaggaac actagtccca
     4081 gccttggaga gagcaggggt accaaggatc ttcctccagt gaaggaccct ggagccctat
     4141 ccagggaggg actgctggcc ccactgggtc tgctggccat cctgaccttg gcagtagcca
     4201 cactgtatgc actatccctg gccacacctg gggagtagtt aaccggtgaa gtaaagcggc
     4261 cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4321 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4381 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4441 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4501 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4561 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4621 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4681 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4741 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4801 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     4861 ggaattctgc agatatccgg ttagtaatga gtttggaatt aattctgtgg aatgtgtgtc
     4921 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4981 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5041 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5101 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5161 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     5221 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     5281 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     5341 ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     5401 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     5461 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     5521 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     5581 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     5641 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     5701 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagcaca
     5761 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     5821 ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc
     5881 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     5941 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6001 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6061 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     6121 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     6181 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6241 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     6301 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6361 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6421 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     6481 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6541 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6601 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     6661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6781 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6841 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6901 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6961 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7021 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7081 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7201 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7261 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     7321 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     7381 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     7441 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     7501 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7561 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7621 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7681 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7741 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7801 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7861 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7921 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7981 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8041 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8101 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     8281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     8341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     8401 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     8461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     8521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     8581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8881 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8941 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9001 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9061 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     9121 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     9181 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     9241 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     9301 aaaagctgga gctgcaagct taa
//
