LOCUS       sequence-32488-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-375
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(466..490)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             516..557
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(597..616)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    641..689
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tggctactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
       61 agacccaagc tggctagtta agctatcaac aagtttgtac aaaaaagcag gctgcggtgc
      121 tcaggtgaga gcggcggcta gcgggagcgc tgtgcactcg aggaagctca tccaccagac
      181 accgccgacg actgccgccc ggccccgggt cttccgctcc ggccccgcga cgagcccctc
      241 gcacaaaccg gacctgagcg ttttgttcgt tcggctcgcg tgaggcaggg gcggcttctc
      301 agcatcagcc ttggggccgg ccagatcgcc atgcaaacac cagccgccgt cgccaccgcc
      361 accatatggg tcgcagagac tgagcacggt ccccccaggg gggagacaac cggttcccat
      421 caggccccgc ctgccctgcc ctcagactct gagctgccag gcataaccca gctttcttgt
      481 acaaagtggt tgatctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc
      541 tcggtctcga ttctacgcgt accggttagt aatgagttta aacgggggag gctaactgaa
      601 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
      661 aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
      721 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
      781 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
      841 aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc acgcgccctg
      901 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc a
//
